Rapid, specific and quantitative analysis of drug candidates in biological samples supports all aspects of ADME lead optimisation at CDCO. LC-MS and LC-MS/MS offer the flexibility to develop specific and rapid methods for the analysis of candidate drugs and their metabolites in complex biological matrices.
Research platforms and equipment include:
- Single quadrupole mass spectrometers—utilised for samples requiring high specificity in a complex matrix, rapid throughput and moderate sensitivity.
- Triple quadrupole mass spectrometers—providing high specificity and sensitivity, primarily used for quantitative analysis of plasma samples from PK studies.
- Time-of-flight (TOF) mass spectrometers—providing high accuracy in mass determination, used to identify metabolites resulting from in vitro and in vivo biotransformation reactions.
- High-performance and ultra-performance liquid chromatography (HPLC/UPLC) systems equipped with plate-based sampling compatibility—providing high throughput method development and validation, sample preparation and processing.
CDCO also utilises a number of conventional HPLC systems with UV and fluorescence detection methods.