New Processes and BioProducts via Enzyme Catalysis
Abstract: Enzymes have a number of remarkable properties that meet the need for sustainable reactions. The action of enzymes is closely tied to the structural traits of their three dimensional protein structure. In the development of sustainable processes and design of biomolecules involving enzyme catalysis there is a continued need for understanding enzymes with respect to substrate selectivity, catalytic mechanism, and structural traits determining the kinetics (catalytic rate), reaction optima and stability traits. The talk will summarize recent results from 3 different applied enzyme catalysis projects: 1. Enzymatic synthesis of human milk oligosaccharides and a discussion of how these processes can provide for value addition to dairy side streams 2. Laccase catalysis on lignin and measurement of the biocatalytic formation of radicals. 3. Kinetics based reaction optimization of enzyme catalysed reduction of formaldehyde to methanol with synchronous cofactor regeneration – a process tied to enzymatic conversion of CO2.
Biography: Professor Anne S. Meyer is Professor and Head of Center for BioProcess Engineering within the Department of Chemical and Biochemical Engineering, Technical University of Denmark (DTU). She holds an MSc from the University of Copenhagen, and an MSc from the University of Reading, UK, plus a PhD from the Technical University of Denmark. She has had two postdoc stays in the USA at Univ. California Davis. In 1998 she was promoted to Associate Professor of Biotechnology/Enzyme Technology at DTU. In 2006 she assumed her current role as Full Professor of BioProcess Engineering and Head of Section BioProcess Engineering, Department of Chemical and Biochemical Engineering, DTU. She is currently visiting professor at Department of Chemical and Biomolecular Engineering, University of Melbourne, Australia. Her research area focuses on design and development of bioprocesses with Biocatalysis Kinetics, Enzyme Technology, Biorefining, Biomass processing and Integrated Separation Technology as core research discipline areas at DTU. Recently expanded to enzymatic CO2 conversion and enzymatic biorefining of marine macroalgae. At the Center of BioProcess Engineering, DTU, she has developed an enzyme production platform for recombinant production of eukaryotic (fungal) and prokaryotic (bacterial) enzymes with Pichia pastoris and E. coli as key work-horse hosts in 5 Liter fermentation systems. Her research is usually done in large collaboration programmes including industrial collaboration both internationally and nationally, and she has PI and leadership experience from several such projects. Distinctions and awards: 2005: Danisco Research Award. 2008: Grundfos Denmark Research Innovation Award. 2011: DTU PhD Supervisor of the Year. She has published more than 275 peer reviewed papers within the field of enzyme catalysis, enzyme kinetics, biomass conversion, biocatalytic carbohydrate synthesis, bioprocess technology and agroindustrial refining technology- she currently has more than 9000 ISI Thomson citations and a Web-of-Science h-index of 51 (Google Scholar: 14700 citations, h-index 65).