Mr. Eugene Obeng

Mr. Eugene Obeng

Doctor of Philosophy Student
Department of Chemical Engineering
Clayton, VIC 3166, Australia

Eugene Marfo Obeng holds a B.Sc Chemical Engineering and M.Sc Biotechnology from the Kwame Nkrumah University of Science and Technology (Ghana) and Universiti Malaysia Sabah (Malaysia), respectively. During the M.Sc studies, Eugene worked on the development of bacterial surface display systems for the depolymerization of lignocellulose. Currently, Eugene works at the Bio Engineering Laboratory of the Department of Chemical Engineering, Monash University (Australia) as a PhD student. Eugene does research in Bio- and Chemical Engineering, where he engineers bifunctional biomolecules for biosensor and bio-imaging applications. His research enables him to explore high-end instrumentation such as electron microscopy and super-resolution light microscopy to elucidate cellular occurrences.

Qualifications

  • Master of Science, Biotechnology, Universiti Malaysia Sabah, Malaysia, 2017
  • Bachelor of Science (Hons), Chemical Engineering, Kwame Nkrumah University of Science and Technology, Ghana, 2013

Expertise

Microscopy

STED microscopy (Abberior Systems), Confocal microscopy (Airyscan LSM 880; Nikon A1, C1) and TEM (FEI T20).

Cell processing

HeLa cells, HEK 293, Pichia pastorisPseudomonas putida and Escherichia coli

Protein expression

Immunogen (VP1), nanobodies, enzymes, fluorescent proteins, intrinsically disordered proteins (IDPs) 

Biomolecular purification

Affinity chromatography (His, GST and MBP), Ion exchange chromatography, and Gel filtration. 

Biomolecular modification

Sortase A transpeptidation, Bioclick chemistry, BirA biotinylationnative chemical conjugations

Biomolecular interaction

Molecule binding and interaction (BLItz, Pall ForteBio LLC), Dynamic light scattering (Zetasizer, Malvern Panalytical) 

Software proficiency

Statistica, Design Expert, Fiji, Microsoft office

Awards

16 Feb 2020: Light Microscopy Australia Travel Grant Award (AU$500)

2018-2021: Faculty of Engineering International Postgraduate ResearchScholarship (FEIPRS), Monash University, Australia.

2018-2021: Co-Funded Monash GraduateScholarship (CF-MGS), Monash Graduate Research Office (MGRO), Australia.

2015-2017: Skim Yuran Pengajian Antarabangsa (SYPA) (Translation: International Study Fee Scheme), Universiti Malaysia Sabah

28 June 2016: Outstanding Progress Presentation Award, 18th Biotechnology Research Institute Postgraduate Seminar, UniversitiMalaysia Sabah, Malaysia.

Associations/Membership

2020-2021: Light Microscopy Australia (LMA), Australia.

2020-2021: American Institute of Chemical Engineers (AIChE)

2020-2021: Australian Microscopy and Microanalysis Society (AMMS), Australia.

2019-2021: Bioprocessing Network (BPN), Australia.

2019-2021: New York Academy of Science (NYAS), USA.

2018-2021: Chemical Engineering Postgraduate Association (CEPA), Monash University, Australia.

 

 

JOURNAL PUBLICATIONS 

  1. Caleb Acquah, Dominic Agyei, Eugene Marfo Obeng, Sharadwata Pan, Kei Xian Tan & Michael Kobina Danquah*. “Aptamers: an emerging class of bioaffinity ligands in bioactive peptide applications”, Critical Reviews in Food Science and Nutrition2019, 4,1-12. Doi: 10.1080/10408398.2018.1564234 
  2. Yi Wei Chan, Caleb Acquah, Eugene M. Obeng, Elvina C. Dullah, Jaison Jeevanandam & Clarence M. Ongkudon. “Parametric study of immobilized cellulase-polymethacrylate particle for the hydrolysis of carboxymethyl cellulose”, Biochimie, 2019, 157, 204-212. Doi: 10.1016/j.biochi.2018.11.019 
  3. Eugene M. Obeng*, Brossette Tatjana, Clarence M. Ongkudon*Cahyo Budiman, Ruth Maas & Joachim Jose. “The workability of Escherichia coli BL21 (DE3) and Pseudomonas putida KT2440 expression platforms with autodisplayed cellulases: A comparison”, Applied Microbiology and Biotechnology, 2018, 1102 (11): 4829-4841. DOI: 10.1007/s00253-018-8987-4 
  4. Eugene M. Obeng*, Clarence M. Ongkudon*, Cahyo Budiman, Ruth Maas & Joachim Jose. “An optimal blend of single autodisplayed cellulases for cellulose saccharification—a proof of concept”, Journal of Chemical Technology & Biotechnology, 93: 2719-2728. DOI: 10.1002/jctb.5628 
  5. Eugene M. Obeng*Cahyo Budiman & Clarence M. Ongkudon*. “Identifying additives for cellulase enhancement—a systematic approach”, Biocatalysis and Agricultural Biotechnology2017, 11, 67-74. DOI: 10.1016/j.bcab.2017.06.006 
  6. Eugene M. Obeng, Siti Nurul Nadzirah Adam, Cahyo Budiman, Clarence M. Ongkudon*, Ruth Maas & Joachim Jose. “Lignocellulases: a review of emerging and developing enzymes, systems and practices“, Bioresources and Bioprocessing, 2017, 4(1)16. DOI: 10.1186/s40643-017-0146-8 
  7. Daniel A. Anang*, Ruth A. Pobee, E. Antwi, Eugene M. Obeng*, Amy AtterFoster K. Ayittey & John T. Boateng. “Nutritional, microbial and sensory attributes of bread fortified with defatted watermelon seed flour”, International Journal of Food Science and Technology2018DOI: 10.1111/IJFS.13727 
  8. Caleb Acquah, Eugene Marfo Obeng, Dominic Agyei, Clarence M. Ongkudon, Charles K. S. Moy & Michael K. Danquah*. “Nano-doped monolithic materials for molecular separation”, Separations, 2017, 4 (2): DOI:10.3390/separations4010002 
  9. Yi Wei Chan, Siti Nurul N. Adam, Eugene M. Obeng, Clarence M. Ongkudon*. “Fabrication of a homogenous large-volume polymethacrylate monolith: A thermal mixing approach”, Separation Science plus, 2018, 1-8. DOI: 10.1002/sscp.201800100 
  10. Eugene Marfo Obeng, Elvina Clarie Dullah, Nur Syahadatain Abdul Razak, Michael K. Danquah, Cahyo Budiman & Clarence M. Ongkudon*. “Elucidating endotoxin-biomolecule interaction with FRET: Extending the frontiers of their supramolecular complexation”, Journal of Biological Methods, 2017, 4(2): e71. DOI: 10.14440/jbm.2017.172 
  11. Eugene Marfo Obeng, Elvina Clarie Dullah, Michael K. Danquah, Cahyo Budiman & Clarence M. Ongkudon*. “FRET spectroscopytowards effective biomolecular probing”, Analytical Methods2016, 8, 5323–5337. DOI:10.1039/C6AY00950F

 BOOK CHAPTER 

  1. Sylvia Y. Edifor, Malcom F. Dapaah, Eugene M. Obeng, Caleb Acquah, Martina F. Baidoo, Clarence M. Ongkudon, Michael K. Danquah*. “Sustainability analysis of oil palm processing: a holistic approach”, In: Impacts and Challenges in Oil Palm Cultivation and Downstream Applications of Biomass (Ed: Mui-Yun Wong), Nova Science Publishing Inc, 2018, 1-36. 

CONFERENCE PROCEDINGS/Presentation 

  1. Eugene M. Obeng, Alex Fulcher, Bhuvana Shanbhag, Ian Harper & Lizhong He. “Spatiotemporal localization of protein droplets in cells”, 26th Australian Conference on Microscopy and Microanalysis, 16th – 20th February 2020. National Conference Centre, Canberra, Australia.
  2. Eugene M. Obeng, Siti Nurul Nadzirah Adam, Cahyo Budiman & Clarence M. Ongkudon. “Additive Enhancing Effect on Cellulases”, Borneo Biotech Symposium, Biotechnology and National Development, 7-8 December 2016, Biotechnology Research Institute, University Malaysia Sabah, Malaysia. 
Last modified: 03/07/2020