Haigh Group - Mammalian Functional Genetics

Group Leader
Assoc Prof Jody Haigh

Senior Research Officers
Dr Catherine Carmichael
Dr Jackie Wang

PhD Student
Mr Mina Takawy

Key terms: Leukaemia mouse models

Research Overview

The laboratory of Associate Professor Jody J. Haigh uses/develops cutting edge transgenic mouse models and ES/iPS cell-based technologies to better understand cellular fate, memory and transformation. The group is presently interested in understanding the role of Zeb and Snai family of transcriptional modulators in normal haematopoietic cell differentiation and lineage commitment/function, as well as their roles in the leukaemia development. Using induced pluripotent stem (iPS) cell based technologies we are also developing iPS cell lines derived from primary leukaemia samples to better understand the molecular basis of leukaemia and to develop novel drug screening platforms.

The group

Haigh Lab
2017. Back row L-R: Ms Anna Milne, Associate Professor Jody Haigh, Dr Katharina Haigh,Mr Mina Takawy,
Front row L-R: Dr Thao Nguyen, Dr Catherine Carmichael,  Dr Jackie Wang, Ms Charlotte De Maziere,
Mr Maximilian Garwood.

Projects

1.    Role of Zeb2 in leukemic Stem Cell Formation and Cancer Progression.
2.    Role of Snai1 in hematopoiesis and leukemia.
3.    Generation of use of leukemic iPS cells to understand human disease.

For current project opportunity descriptions please visit our honours page.

Projects and Grant Funding

Our work is funded by the NHMRC.

Publications

Zeb and Snai related publications:

Oncogenic ZEB2 activation drives sensitivity towards LSD1 inhibition in T-cell acute lymphoblastic leukemia. Goossens S*, Peirs S*, Van Loocke W, Takawy M, Sonderegger S, Haigh K,  Ngyuen T, Vandamme N, Costa M, Carmichael C, Van Nieuwerburgh F, Deforce D, Kleifeld O, Curtis D, Berx G, Van Vlierberghe P* and Haigh J.J.* Blood Journal. 2017, Feb 23. 129(8): 981-990.

The Snail family in normal and malignant hematopoiesis. Carmichael C and Haigh J.J. Cells, Tissues, Organs (Review) 2017, Feb 1. 203(2): 82-98.

The EMT transcription factor controls adult murine hematopoietic differentiation by regulating cytokine signaling. Riedt T*, Goossens S*, Li J, García C.C, Dobrosch L, Gütgemann I, Radaelli E, Fröhlich H, Huylebroeck D, Brossart P, Haigh J.J, and Janzen V. Blood Journal, 2017, Jan 26. 129(4): 460-472.

Transcriptional repressor ZEB2 promotes terminal differentiation of CD8+ effector and memory T cell populations during infection. Omilusik, K.D., Best, J.A., Yu, B., Goossens, S., Weidemann, A., Nguyen, J.V., Seuntjens, E., Stryjewska, A., Zweier, C., Roychoudhuri, R., Gattinoni, L., Bird, L.M., Higashi, Y., Kondoh, H., Huylebroeck, D., Haigh, J.J., Goldrath, A.W. J. Exp. Med. 2015, Oct.26, 202(12): 2027-2039

Terminal NK cell maturation is controlled by concerted actions of T-bet and Zeb2 and is essential for melanoma rejection. van Helden, M.J., Goossens, S., Daussy, C., Mathieu, A.L., Faure, F., Marçais, A., Vandamme, N., Farla, N., Mayol, K., Viel, S., Degouve, S., Debien, E., Seuntjens, E., Conidi, A., Chaix, J., Mangeot, P., de Bernard, S., Buffat, L., Haigh, J.J., Huylebroeck, D., Lambrecht, B.N., Berx, G., Walzer, T. J. Exp. Med. (2015) Oct.26, 212(12): 2015-2025

ZEB2 drives immature T-cell lymphoblastic leukaemia development via altered IL-7 receptor signalling and enhanced tumor-initiating potential.  Goossens, S., Radaelli, E., Blanchet, O., Durinck, K., Van der Meulen, J., Peirs, S., Taghon, T., Tremblay, C., Costa, M., Farhang Ghahremani, M., De Medts, J., Bartunkova, S., Haigh, K., Schwab, C., Farla, N., Pieters, T., Matthijssens, F., Van Roy, N., Best, J.A., Deswarte, K., Bogaert, P., Carmichael, C., Rickard, A., Suryani, S., Bracken, L.S., Alserihi, R., Canté-Barrett, K., Haenebalcke, L., Clappier, E., Rondou, P., Slowicka, K., Huylebroeck, D., Goldrath, A.W., Janzen, V., McCormack, M.P., Lock, R.B., Curtis, D.J., Harrison, C., Berx, G., Speleman, F., Meijerink, J.P.P., Soulier, J., Van Vlierberghe, P. and Haigh, J.J. Nature Communications, 2015, Jan 7; 6:5794

Snai1 regulates cell lineage allocation and stem cell maintenance in the mouse intestinal epithelium. Horvay, K., Jarde, T., Casagranda F., Perreau V.M., Haigh, K., Gridley, T., Berx, G., Haigh, J.J., Barker, N., Polo, J.M., Hime, G.R., Abud, H.E. EMBO Journal, 2015, 12;34(10):1319-35

The EMT regulator Zeb2/Sip1 is essential for murine embryonic hematopoietic stem/progenitor cell differentiation and mobilization. Goossens, S.; Janzen, V.; Bartunkova, S.; Yokomizo, T.; Drogat, B.; Crisan, M.; Haigh, K.; Seuntjens, E.; Umans, L.; Riedt, T.; Bogaert, P.; Haenebalcke, L.; Berx, G.; Dzierzak, E.; Huylebroeck, D.; Haigh, J. J.  Blood Journal, 2011, 117(21):5620-30.

Key technology resource publications:
The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (De)differentiation. Haenebalcke, L.; Goossens, S.; Dierickx, P.; Bartunkova, S.; D'Hont, J.; Haigh, K.; Hochepied, T.; Wirth, D.; Nagy, A.; Haigh, J. J.  Cell Rep, 2013, 3(2):335-41

Efficient ROSA26-Based Conditional and/or Inducible Transgenesis Using RMCE-Compatible F1 Hybrid Mouse Embryonic Stem Cells. Haenebalcke, L.; Goossens, S.; Naessens, M.; Kruse, N.; Ghahremani, M. F.; Bartunkova, S.; Haigh, K.; Pieters, T.; Dierickx, P.; Drogat, B.; Nyabi, O.; Wirth, D.; Haigh, J. J.   Stem Cell Rev, 2013, 9(6):774-85

Efficient mouse transgenesis using Gateway-compatible ROSA26 locus targeting vectors and F1 hybrid ES cells. Nyabi, O.; Naessens, M.; Haigh, K.; Gembarska, A.; Goossens, S.; Maetens, M.; De Clercq, S.; Drogat, B.; Haenebalcke, L.; Bartunkova, S.; De Vos, I.; De Craene, B.; Karimi, M.; Berx, G.; Nagy, A.; Hilson, P.; Marine, J. C.; Haigh, J. J.  Nucleic Acids Res, 2009, 37(7): e55

For full publication list see: https://scholar.google.com.au/citations?user=K4KNEWsAAAAJ&hl=en&oi=ao