Hamilton Group - Platelets & Thrombosis

Justin Hamilton

Group Leader
Associate Professor Justin Hamilton

Research Fellow
Dr Shauna French

Visiting Research Fellow
Dr Niklas Boknas

PhD Students
Ms Maria Selvadurai
Mr Mitchell Moon

Research Assistant
Ms Rachael Canfield

BMSc Honours Student
Mr Roberto Christanto

Honours Student
Mr Michael Zhu

Key terms: Discovering novel anti-thrombotics

Our group

Hamilton lab

2018: L-R: Back row - A/Prof Justin Hamilton, Dr Niklas Boknas. Front row: Mr Michael Zhu, Dr Shauna French, Ms Rachael Canfield, Mr Mitch Moon. Absent: Ms Maria Selvadurai, Mr Roberto Christanto

Research goal

To understand platelet activation mechanisms in order to rationalise the development of novel anti-platelet agents for the prevention of arterial thrombosis.

Research Overview

Arterial thrombosis, manifesting predominantly as acute myocardial infarction or ischaemic stroke, is by far the most common cause of death and disability in Australia. Activated platelets are the essential cellular component of arterial thrombi and anti-platelet drugs are the mainstay of current pharmacotherapy for the prevention of arterial thrombosis. Consequently, there is substantial need for novel strategies to inhibit platelet function for the prevention of heart attack and stroke. Our research examines the cell biology of blood platelets and their precursor cell, the megakaryocyte, in order to understand the mechanisms by which platelets are produced and function, since examining the mechanisms of platelet production and function is likely to reveal novel approaches for pharmaceutical platelet inhibition.

Heart Foundation

A/Prof Justin Hamilton is a senior researcher at the ACBD who has received significant research support from the Heart Foundation over the past decade. Listen to his account of the current research he is undertaking, why he got into cardiovascular research and what you can do to help support the Heart Foundation in its ongoing fight against cardiovascular disease. (Videos courtesy the Heart Foundation)

Video formats:

Windows media (WMV, 8.39 MB)
Quicktime Video (mp4 8 MB)

Projects

  1. Defining the function of the thrombin receptor, PAR4, on human platelets.
  2. Investigating the role of Class II PI3Ks in platelet production and function.

For current project opportunity descriptions please visit our honours page, or contact Justin Hamilton directly.

Projects and Grant Funding

Our work is funded by the ARC, NHMRC, NHFA and the CASS Foundation.

  • Title: Examining novel cell signalling in the regulation of platelet structure and function.
    Investigators: Hamilton, J
    Funding: (2014 - 2017). Australian Research Council (ARC).
  • Title: Defining the function of the thrombin receptor, PAR4, on human platelets.
    Investigators: Hamilton, J
    Funding: (2013 - 2015). National Health & Medical Research Council (NHMRC).
  • Title: Nikon AIR Confocal Microscope.
    Investigators: Jackson, S, Medcalf, R, Mackay-Fisson, F, Plebanski, M, Andrews, R, Jane, S, Curtis, D, Morganti-Kossmann, M, Peter, K, Chin-Dusting, J, Mak, J, Pereira, C, Hamilton, J, Cody, S
    Funding: (2011 - 2015). National Health & Medical Research Council (NHMRC).

Recent Publications

  • Zheng Z, Pinson J-A, Mountford S.J, Orive S, Schoenwaelder S.M, Shackleford D, Powell A, Nelson E.M, Hamilton J.R, Jackson S.P, Jennings J.G, Thompson P.E. Discovery and antiplatelet activity of a selective PI3Kβ inhibitor (MIPS-9922). European Journal of Medicinal Chemistry, 2016:122, 339-351
  • French S.L, Paramitha A.C, Moon M.J, Dickins R.A, Hamilton J.R. Humanizing the protease-activated receptor (PAR) expression profile in mouse platelets by knocking Par1 into the Par3 locus reveals Par1 expression is not tolerated in mouse platelets. PLoS One, 2016:11(10) Article number e0165565
  • French S.L, Arthur J.F, Lee H, Nesbitt W.S, Andrews R.K, Gardiner E.E, Hamilton J.R. Inhibition of protease-activated receptor 4 impairs platelet procoagulant activity during thrombus formation in human blood. Journal of Thrombosis and Haemostasis, 2016:14(8) 1642-1654
  • Petitjean C, Setiabakti C.M, Mountford J.K, Arthur J.F, Ellis S, Hamilton J.R. Combined deficiency of PI3KC2α and PI3KC2β reveals a nonredundant role for PI3KC2α in regulating mouse platelet structure and thrombus stability. Platelets, 2016:27(5) 402-409
  • Mountford J.K, Petitjean C, Putra H.W.K, McCafferty J.A, Setiabakti N.M, Lee H, Tonnesen L.L, McFadyen J.D, Schoenwaelder S.M, Eckly a, Gachet C, Ellis S, Voss A.K, Dickins R.A, Hamilton J.R, Jackson S.P. The class II PI 3-kinase, PI3KC2α, links platelet internal membrane structure to shear-dependent adhesive function. Nature Communications, 2015:6, Article number 6535