BD FACSCanto II

About the BDBiosciences FACSCanto II analyser

The BD FACSCanto II analyser is equipped with 3 lasers, 2 light scatter detectors and 8 fluorescence detectors.
The instrument is easy to startup and does not require daily laser alignment.  Samples can be run on this instrument at a maxiumum of 10,000 cells/second.

At a glance

  • 405nm, 488nm, 635nm excitation
  • 8 fluorescence detectors
  • FACSDiva software

In the table below are some of the common fluorochromes that can be used in conjunction on this instrument:

LaserDetectorFiltersCommon Fluorochromes
488nm FL1 530/30 FITC
Alexa Fluor 488
EGFP
CFSE
FL2 585/42 PE
FL3 670LP Pe-Cy5
PE-Cy5.5
PI
PerCP
PerCP-Cy5.5
Qdot705
FL4 780/60 PE-Cy7
635nm FL5 660/20 APC
Alexa Fluor 647
FL6 780/60 APC-Cy7
APC-Alexa Fluor 750
405nm FL7 450/50 Pacific Blue
FL8 510/50 AmCyan

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