Beckman Coulter MoFlo BTA

About the Beckman Coulter MoFlo BTA analyser

The Beckman Coulter MoFlo BTA is a high speed cell analyser.  It is equipped with 2 lasers, 2 light scatter detectors and 5 fluorescence detectors. The instrument uses a 100nm nozzle and runs at a pressure of 35psi.  With this setup, it is capable of running samples at speeds of up to 15,000 cells/second.

At a glance

  • 488nm, 635nm excitation
  • 5 fluorescence detectors
  • Summit software

In the table below are some of the common fluorochromes that can be used in conjunction on this instrument:

LaserDetectorFiltersCommon Fluorochromes
488nm FL1 530/40 FITC
Alexa Fluor 488
EGFP
CFSE
FL2 570/70 PE
FL3 670/40 or
700/30
PE-Cy5
PE-Cy5.5
PI
Qdot 655
Qdot 705
633nm FL4 740LP APC-Cy7
APC-AlexaFluor 750
FL5 670/40 APC
Alexa Fluor 647

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