Beckman Coulter MoFlo SRT

About the Beckman Coulter MoFlo SRT cell sorter

The Beckman Coulter MoFlo SRT is a high speed cell sorter.  It is equipped with 3 lasers, 2 light scatter detectors and 6 fluorescence detectors.

Up to 2-way sorts are available and samples can be sorted into 12x75mm FACS tubes, 1.5mL eppendorfs, 15mL centrifuge tubes, 50mL centrifuge tubes.

The instrument uses a 90nm nozzle and runs at a pressure of 35psi.  With this setup, it is capable of running samples at speeds of up to 20,000 cells/second.

At a glance

  • UV, 488nm, 633nm excitation
  • 6 fluorescence detectors
  • 2-way sorting
  • Summit software

In the table below are some of the common fluorochromes that can be used in conjunction on this instrument:

LaserDetectorFiltersCommon Fluorochromes
488nm    FL1 530/40 FITC
Alexa Fluor 488
EGFP
CFSE
FL2 570/70 PE
FL3 670/40 or
700/30
PE-Cy5
PE-Cy5.5
PI
Qdot 655
Qdot 705
633nm FL4 670/40 APC
Alexa Fluor 647
UV FL5 450/65 Hoechst 33342 - Blue
DAPI
Indo-1
FL6  630/30 Hoechst 33342 - Red

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