Beckman Coulter MoFlo XDP

About the Beckman Coulter Mo-Flo XDP cell sorter

The Beckman Coulter MoFlo XDP is a high speed cell sorter.  It is equipped with 3 lasers, 2 light scatter detectors and 11 fluorescence detectors.  The instrument is equipped with a temperature controlled waterbath to keep samples and collected samples at a required temperature (2C - 37C).  An aerosol containment unit with filter is also attached for operator safety.

Up to 4-way sorts are available and samples can be sorted into plates (1536-, 384-, 96-, 24-, wells), 12x75mm FACS tubes, 1.5mL eppendorfs, 15mL centrifuge tubes (2-way sort only), 50mL centrifuge tube (2-way sort only) and onto standard slides.

The instrument uses a 70nm nozzle and runs at a pressure of 60psi.  With this setup, it is capable of running samples at speeds of up to 40,000 cells/second.

At a glance

  • 405nm, 488nm, 635nm excitation
  • 11 fluorescence detectors
  • 4-way sorting
  • Single cell deposition
  • Temperature control
  • Summit Software

In the table below are some of the common fluorochromes that can be used in conjunction on this instrument:

Laser Detector FiltersCommon Fluorochromes
488nm      FL1 530/40 FITC
Alexa Fluor 488
EGFP
CFSE
FL2 580/30 PE
FL3 613/20 PE-Texas Red
QDot 605
FL4 670/40 or
700/30
Pe-Cy5
PE-Cy5.5
PI
Qdot 655
Qdot 705
FL5 740LP PE-Cy7
405nm    FL6 450/65 Pacific Blue
FL7 560/40 Pacific Orange
FL8 670/40 Qdot 655
635nm    FL9 670/30 APC
Alexa Fluor 647
FL10 720/45 Alexa Fluor 700
FL11 780/40 APC-Cy7
APC-Alexa Fluor 750

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