Cyclic peptides nanotubes (CPNT) for gene delivery

We have developed a new range of nanomaterials, based on the self-assembly of cyclic peptides into nanotubular structures, with great potential for gene delivery. Cyclic peptide nanotubes (CPNT) can penetrate lipid membranes and create a pore in the bilayer for direct injection. This unique way of interaction makes them excellent candidates to beat the current transfecting agents in gene therapy applications. This Honours project will focus on the synthesis of a library of siRNA-CPNT polyplexes with novel architectures, quantify their efficiency in gene transfection and establish the mechanism of endosomal escape of the aggregates. The project will evolve into applying this system to the repression of certain important genes in mammalian cell line models.

This Honours project will focus on the synthesis of a library of siRNA-CPNT polyplexes with novel architectures, quantify their efficiency in gene transfection and establish the mechanism of endosomal escape of the aggregates. The project will evolve into applying this system to the repression of certain important genes in mammalian cell line models.