Understanding the spatial organisation of GPCR signalling
Cellular responses are encoded by highly organised intracellular signals initiated from the plasma membrane. This signalling can occur in very small domains of the cell (plasma membrane, endosomes, nucleus) and is regulated in space and time. The lab is interested in understanding how these signals are initiated and controlled by G protein-coupled receptors, and how they can be dysregulated during diseases such as cancer, to drive metastatic progression. Available projects examine:
1. Dysregulation of GPCR signalling in breast cancer
2. Manipulation of spatial signalling using optogenetics (light-activated GPCRs)
3. Development of novel imaging methods to measure spatial signalling
4. Unique spatial signalling by ultra-low concentrations of GPCR ligands Techniques frequently used in the lab include cell culture, confocal and high-content imaging, signalling and invasion assays, western blotting and proteomics.