HPLC Workshops

Due to COVID-19, note this workshop is currently unavailable until further notice.

For Year 11 and 12 Chemistry

In this practical, students get to use high-performance liquid chromatography instruments to create a calibration curve and determine the concentration of caffeine in unknown samples.  Students will be taught how to operate the instruments, manipulate the data in the HPLC software and then will design their own experiment.  This workshop is a fantastic opportunity for students to consolidate their understanding of chromatography, create their own data sets and apply the principles of the scientific method.


  • Monash University, Faculty of Pharmacy and Pharmaceutical Sciences, 381 Royal Parade, Parkville, 3052


  • 3 hours


  • Best for a maximum of 25 students (20 and fewer is recommended)


  • Flexible with days and times, subject to instrument and demonstrator availability

Concepts explored

It is recommended that students have familiarity with the below concepts prior to the workshop.

  • The adsorption and solubility of compounds based on relative polarity in different phases
  • The Rf value and Retention time
  • Interpreting chromatograms
  • Constructing of a calibration/standard curve
  • The interpretation of a calibration/standard curve to find the concentration of an unknown sample

Key skills developed

  • Use of HPLC instrument hardware and software
  • The construction of a key question, aim and hypothesis when designing an experiment
  • The design of a fair test
  • The interpretation of graphical results
  • The identification of errors during the experimental process
Interested?  Contact us to find out more or book in a workshop for your class.