Beckman Coulter CytoFLEX

  • Lasers 3: 405nm, 488nm, 640nm
  • Detectors: 13 fluorescent channels
  • Sample tubes: 5ml Polystyrene or Polypropylene
  • Plate Loader: up to 96 well plates
  • Software: CytExpert
  • Small particle detector: 405nm SSC
  • BC CytoFLEX Detector Guide

Laser Wavelength

Filter

Frequently Used Fluorochromes

Blue 488nm

525/40

FITC, GFP, A488, CFSE, BB515, Fluo-3

 

585/42

PE, PI

 

610/20

ECD, PE-TxRed, PI

 

690/50

PerCP, PerCP-Cy5.5, 7AAD, PI

 

780/60

PE-Cy7, PE-Vio 770, PE-A750, QD800

Red 640nm

660/10

APC, A647, Sytox Red, eF660

 

712/25

A700, A680

 

780/60

APC-Cy7. APC-eF780, APC-Fire 780, NIR

Violet 405nm

450/45

BV421, Pac Blue. Sytox Blue, e450

 

525/40

BV500, BV510, Aqua, Pac Orange

 

610/20

V610, BV605, QD605

 

660/10

V660, BV650, QD655

 

780/60

V780, BV786, QD900