Surface Plasmon Resonance (SPR) is a biosensor technology enabling label-free and real- time measurement of protein-protein interactions. In the capture assay, the ligand of interest binds to the specific antibody that is immobilized on a chip. The kinetics of binding can then be measured. The principle uses of SPR analysis are to identify complimentary binding pairs of antibodies with recombinant antigen or to determine binding kinetics and affinity of antibodies with the parent antigen.
SPR analysis is run in partnership with the Monash Fragment Platform