MB Course - Lecture Series
The following broad topics are covered in a series of 10 lectures
throughout the week.
throughout the week.
- Introduction to Microbiology, DNA and Molecular Biology
- Basic Cloning Requirements
- Gene Cloning Techniques
- Molecular Hybridisation (Northern, Southern and Western)
- Construction and screening of Genomic and cDNA Libraries
- Restriction Fragment Mapping
- Design and use of Oligonucleotide Primers
- Genetic Mapping
- DNA Sequencing
- Polymerase Chain Reaction and Real-Time PCR (qPCR)
- Site-directed Mutagenesis
- Cloning Vectors and their Applications
- Gene Expression Systems
- Bioinformatics and Database Searching