MB Course - Lecture Series

The following broad topics are covered in a series of 10 lectures
throughout the week.
  • Introduction to Microbiology, DNA and Molecular Biology
  • Basic Cloning Requirements
  • Gene Cloning Techniques
  • Molecular Hybridisation (Northern, Southern and Western)
  • Construction and screening of Genomic and cDNA Libraries
  • Restriction Fragment Mapping
  • Design and use of Oligonucleotide Primers
  • Genetic Mapping
  • DNA Sequencing
  • Polymerase Chain Reaction and Real-Time PCR (qPCR)
  • Site-directed Mutagenesis
  • Cloning Vectors and their Applications
  • Gene Expression Systems
  • Bioinformatics and Database Searching