MB Course - Practical Applications
The following applications are covered in the practical laboratory work spanning
30 hours of the workshop.
- Cleavage and Ligation of Plasmid DNA
- Transformation of Bacterial Cells
- Preparation and Purification of Plasmid DNA
- Restriction Enzyme Mapping
- Agarose Gel Electrophoresis
- Recovery of DNA from Agarose Gels
- Preparation of Probes
- DIG-based Southern Blotting and Hybridisation
- Induction of Fusion Protein Production
- Polyacrylamide Gel Electrophoresis and Western Blotting
- Nucleic Acid Extraction
- Reverse Transcription and PCR
- Quantification of DNA and RNA by Bioanalysis
- Real-time PCR (qPCR)
- Sanger DNA Sequencing and Computer-aided Analysis