MB Course - Practical Applications

The following applications are covered in the practical laboratory work spanning
30 hours of the workshop.

  • Cleavage and Ligation of Plasmid DNA
  • Transformation of Bacterial Cells
  • Preparation and Purification of Plasmid DNA
  • Restriction Enzyme Mapping
  • Agarose Gel Electrophoresis
  • Recovery of DNA from Agarose Gels
  • Preparation of Probes
  • DIG-based Southern Blotting and Hybridisation
  • Induction of Fusion Protein Production
  • Polyacrylamide Gel Electrophoresis and Western Blotting
  • Nucleic Acid Extraction
  • Reverse Transcription and PCR
  • Quantification of DNA and RNA by Bioanalysis
  • Real-time PCR (qPCR)
  • Sanger DNA Sequencing and Computer-aided Analysis