DNA Sequencing

The core application of the Micromon NGS facility is DNA sequencing. This can be used for resequencing, de novo assembly of genomes or other genetic elements, gene discovery, identification of genome variation (SNP identification, genome rearrangements, insertions and deletions), and more.

We can produce single or paired reads with read lengths ranging from 50 to 150 bases (and soon up to 450b). Insert sizes for paired-end sequencing can range from 200b to  5kb (we can produce 10kb and 20kb insert sizes for special circumstances).

How to use our NGS DNA Sequencing service

Simply download the Sample Submission Form, fill it in, and email it back to us. Then, deliver your sample labelled as per your submission form. We'll enter your sample into our QC process and commence library preparation. You will receive an email when we receive your sample, when your sample is ready for sequencing, and when your sequencing is complete. Finally, when your data is ready, we will send you the details required to download your data from your dedicated result page on our website.

What to submit:

  • a completed sample submission form
  • 2ug of DNA (for eukaryotic organisms)
  • 5ng of DNA (for prokaryotic organisms, viruses, plasmids, etc.)
  • an image of an agarose gel showing your DNA, or a Bioanalyzer trace.

Further details can be found in our Sample Submission Guidelines for DNA sequencing... or just contact us to discuss your project requirements!

Forms and Resources

Delivery and contact details

Sample preparation and submission guidelines

Sample submission form

Whole genome sequencing overview (Illumina)