RNA-seq, or transcriptomics, is a fast and cost-effective method to simultaneously quantitate and obtain the full-length sequence of RNA transcripts. It provides a detailed insight into the type and number of transcripts present in a tissue or population of cells (even without prior knowledge of the genome). Direct sequencing of transcripts with paired-end sequencing can also reveal splice variants. We can process both eukaryotic and prokaryotic mRNA-seq, high and low quality RNA (such as FFPE or otherwise degraded RNA), using inputs from as low as a few nanograms. We're happy to discuss your project further by phone, email or in person.
How to use our Transcriptomics service
Simply download the Sample Submission Form, fill it in, and send it back to us with your samples. We will first estimate the concentration (using spectrophotometry and RNA-specific fluorimetry) and quailty (using spectrophotometry and Agilent Fragment Analyzer) to help us determine the appropriate assay to use. We'll contact you to discuss the results, and proceed to library construction and sequencing. Finally, your data will be delivered via our website, along with sample and data QC reports.
What to submit:
- A completed sample submission form
- 1ng to 5ug of total RNA (see our Sample Submission Guidelines)
- Any QC information you have already obtained (such as an agarose gel image, concentration measurements, a Bioanalyzer/Fragment Analyzer/TapeStation trace, etc).