Interpret and Troubleshoot Sequencing Data
An excellent outline of the basic concepts of Sanger Sequencing is provided in Chapter 1 of the Applied Biosystems Chemistry Guide (3rd edition, 2016):
For a concise coverage of the most common sequencing problems you are likely to encounter, we recommend our guide Common reasons for DNA Sequencing failure
For a much more comprehensive coverage, please see Chapter 8 in the Applied Biosystems Chemistry Guide (3rd edition, 2016)
We have created additional guides which might prove useful in overcoming technical sequencing problems.
Overcoming problems with short mononucleotide repeats (This 2010 research paper from the journal BioTechniques outlines a fusion enzyme methodology to improve sequence data generated from PCR products that contain mononucleotide repeats of 15 bases or less.)