DNA/RNA Quantitation

Quant-IT for Fluorometric DNA Measurements

Fluorescent DNA quantitation utilises a double-stranded DNA specific fluorescent dye. We use this dye in conjunction with a fluorometer (DeNovix DS11-fx or Invitrogen Qubit) to measure the concentration of DNA in your sample. The double-stranded DNA specificity makes this assay more accurate than measurements based on spectrophotometry, such as those obtained from a Nanodrop or similar instrument. The spectrophotometric measurements will detect any components of your sample that absorb at the same wavelength as DNA (usually 260nm is usually used) - such 'contaminants' include PCR primers, nucleotides, RNA, phenol (sometimes used to remove protein from DNA preparations), thiocyanates (component of many commercial 'clean up' kits) and many others. It also allows the measurement of much smaller amounts of DNA - down to 25 pg/mL.

Quant-IT for Fluorometric RNA Measurements

The Quant-IT RNA Reagent is a highly sensitive fluorescent dye that binds to RNA, and is highly selective for RNA over double-stranded DNA. We use this dye in conjuction with a fluorometer to measure the concentration of DNA in your sample. The nucleic acid specificity makes this assay more accurate than measurements based on spectrophotometry, such as those obtained from a Nanodrop or similar instrument. The spectrophotometric measurements will detect any components of your sample that absorb at the same wavelength as RNA - such 'contaminants' include PCR primers, nucleotides, DNA, phenol (sometimes used to remove protein from DNA preparations), thiocyanates (component of many commercial 'clean up' kits) and many others. It also allows the measurement of much smaller amounts of RNA - down to 250pg/uL.

Fluorometric Measurement Workflow

After your online iLab submission and samples have been received, we will first estimate the concentration (using spectrophotometry) to help us determine the appropriate assay to use. We will then measure your sample fluorometrically and prepare a report containing the relevant data. This will be emailed to you using the details you included in your online iLab submission.

Pricing

Quantitation of nucleic acids by fluorometry incurs a one-off setup fee per batch of samples of each sample type. If RNA and DNA samples are delivered together, then all RNA samples will be regarded as one batch (and incur a DNA setup fee) and all DNA samples will be regarded as an additional batch (and incur an RNA setup fee).

Sample TypeMeasurement RangeSetup Fee

Price (per sample)

DNA 2 ng to 1000 ng $4.00 $2.00
DNA 200 pg to 100 ng $4.00 $2.00
RNA 5 ng to 100 ng $4.00 $3.00