Our team provides expertise and training across a wide range of analytical microscopy modalities in the biomedical and life sciences. Ranging from sample preparation and labelling to performing live cell experiments for cell signalling and organ/organism development, we provide guidance and training to allow scientists and graduate students to undertake cutting edge analytical research with confidence.
MMI works closely with other Research Platforms such as Histology, Cryo-EM and eResearch to provide integrated microscopy support to a large research community.
We will guide and train you to perform experiments, produce high quality images and extract analytical data.
Advanced light and fluorescence microscopy
Our instrumentation provides a solid platform of advanced light and fluorescence microscopy techniques, including automation, high-speed imaging, time-lapse, slide scanning (in conjunction with our Histology Patform), tiling and live cell imaging on slides, chambers or microplates. Both upright and inverted instruments are available, and all systems are supported by a comprehensive range of professional software for bioimage analysis to provide quantitative results.
Live cell imaging is one of our specialities
Most of our instruments are equipped with live cell incubators, specialised cell chambers or multiwell plates that support live and long term imaging experiments. Staff have extensive knowledge in experimental design, labelling and analysis.
Optical sectioning and 3D analysis
Our range of instrument modalities for 3D include confocal (laser scanning and spinning disk), multiphoton and lightsheet microscopes. Resonant imaging confocal and lightsheet instruments are capable of high speed imaging. The multiphoton and lightsheet instruments are ideal for deeper tissue imaging in live, fixed or cleared samples, with specialised lone working distance objectives capable of imaging to a depth of 2-6mm (confocal or multiphoton) or even 10mm (ultramicroscope).
Special methods and emerging technologies
Specialised techniques are provided by staff collaboration on our more novel or complex instruments: these include Fluorescence Lifetime Imaging Microscopy (FLIM), Lattice Lightsheet Microscopy, single molecule localisation microscopy (dSTORM, N-STORM), Stimulated Emission Depletion Microscopy (STED), Structured Illumination Microscopy (N-SIM), birefringence microscopy and, in conjunction with the Cryo-EM Platform, correlative light and electron microscopy.
Image analytics and data handling
Extracting and understanding bioimaging data is crucial, and handling big datasets is often a bottleneck in research. Our bioimage analysis team is available to train scientists and students in a range of analytical software we support (ImageJ/ FIJI, Imaris, Huygens, Metamorph). We also develop workflows and new analytical methods for our users. In conjunction with eResearch, we are establishing data handling and analysis pipelines to facilitate the flow of (big)data from instrument to computational workspaces and ultimately to publication.