Publications & Links

General information:

• MPMF Flyer
• Terms & Conditions
• Sample Preparation Guidelines

Registration forms:

• User Registration Form
• Project Registration Form
• Proteomics Request Form (for existing fee-for-service customers)

iLab:

• Register with Monash iLab here
• Need help? Check out our iLab Registration Manuals for Monash PIs, Monash Internal Users or External Users

Software:

• LFQ Analyst
• Visualising crosslinking data (under construction)
• Performance monitors (under construction)

Selected Publications:

2020:

• Spliced Peptides and Cytokine-Driven Changes in the Immunopeptidome of Melanoma.
  Faridi P, Woods K, Ostrouska S, Deceneux C, Aranha R, Duscharla D, Wong SQ, Chen W, Ramarathinam SH, Lim Kam Sian TCC, Croft NP, Li C, Ayala R, Cebon JS, Purcell AW, Schittenhelm RB, Behren A.
  Cancer Immunol Res. 2020 Sep 16. doi: 10.1158/2326-6066.CIR-19-0894.
• SMOC1 is a glucose-responsive hepatokine and therapeutic target for glycemic control.
  Montgomery MK, Bayliss J, Devereux C, Bezawork-Geleta A, Roberts D, Huang C, Schittenhelm RB, Ryan A, Townley SL, Selth LA, Biden TJ, Steinberg GR, Samocha-Bonet D, Meex RCR, Watt MJ.
  Sci Transl Med. 2020 Sep 2;12(559):eaaz8048. doi: 10.1126/scitranslmed.aaz8048.
• Mitochondrial dysfunction caused by outer membrane vesicles from Gram-negative bacteria activates intrinsic apoptosis and inflammation.
  Deo P, Chow SH, Han ML, Speir M, Huang C, Schittenhelm RB, Dhital S, Emery J, Li J, Kile BT, Vince JE, Lawlor KE, Naderer T.
  Nat Microbiol. 2020 Aug 17. doi: 10.1038/s41564-020-0773-2
• Redesign of substrate selection in glycopeptide antibiotic biosynthesis enables effective formation of alternate peptide backbones.
  Kaniusaite M, Kittila T, Goode RJA, Schittenhelm RB, Cryle MJ.
  ACS Chem Biol. 2020 Aug 14. doi: 10.1021/acschembio.0c00435.
• Restriction of essential amino acids dictates the systemic metabolic response to dietary protein dilution.
  Yap YW, Rusu PM, Chan AY, Fam BC, Jungmann A, Solon-Biet SM, Barlow CK, Creek DJ, Huang C, Schittenhelm RB, Morgan B, Schmoll D, Kiens B, Piper MDW, Heikenwälder M, Simpson SJ, Bröer S, Andrikopoulos S, Müller OJ, Rose AJ.
  Nat Commun. 2020 Jun 9;11(1):2894. doi: 10.1038/s41467-020-16568-z.
• Cellular and Structural Basis of Synthesis of the Unique Intermediate Dehydro-F 420-0 in Mycobacteria.
  Grinter R, Ney B, Brammananth R, Barlow CK, Cordero PRF, Gillett DL, Izoré T, Cryle MJ, Harold LK, Cook GM, Taiaroa G, Williamson DA, Warden AC, Oakeshott JG, Taylor MC, Crellin PK, Jackson CJ, Schittenhelm RB, Coppel RL, Greening C.
  mSystems. 2020 May 19;5(3):e00389-20. doi: 10.1128/mSystems.00389-20.
• A Natural Peptide Antigen within the Plasmodium Ribosomal Protein RPL6 Confers Liver T RM Cell-Mediated Immunity against Malaria in Mice.
  Valencia-Hernandez AM, Ng WY, Ghazanfari N, Ghilas S, de Menezes MN, Holz LE, Huang C, English K, Naung M, Tan PS, Tullett KM, Steiner TM, Enders MH, Beattie L, Chua YC, Jones CM, Cozijnsen A, Mollard V, Cai Y, Bowen DG, Purcell AW, La Gruta NL, Villadangos JA, de Koning-Ward T, Barry AE, Barchet W, Cockburn IA, McFadden GI, Gras S, Lahoud MH, Bertolino P, Schittenhelm RB, Caminschi I, Heath WR, Fernandez-Ruiz D.
  Cell Host Microbe. 2020 Jun 10;27(6):950-962.e7. doi: 10.1016/j.chom.2020.04.010.
• Understanding the early stages of peptide formation during the biosynthesis of teicoplanin and related glycopeptide antibiotics.
  Kaniusaite M, Tailhades J, Kittilä T, Fage CD, Goode RJA, Schittenhelm RB, Cryle MJ.
  FEBS J. 2020 May 2. doi: 10.1111/febs.15350.
• Interaction of Plasmodium falciparum casein kinase 1 with components of host cell protein trafficking machinery.
  Batty MB, Schittenhelm RB, Dorin-Semblat D, Doerig C, Garcia-Bustos JF..
  IUBMB Life. 2020 Jun;72(6):1243-1249. doi: 10.1002/iub.2294.
• A Chemoenzymatic Approach to the Synthesis of Glycopeptide Antibiotic Analogues.
  Tailhades J, Zhao Y, Ho YTC, Greule A, Ahmed I, Schoppet M, Kulkarni K, Goode RJA, Schittenhelm RB, De Voss JJ, Cryle MJ.
  Angew Chem Int Ed Engl. 2020 Jun 26;59(27):10899-10903. doi: 10.1002/anie.202003726
• Phosphoproteomic characterization of the signaling network resulting from activation of the chemokine receptor CCR2.
  Huang C, Foster SR, Shah AD, Kleifeld O, Canals M, Schittenhelm RB, Stone MJ.
  J Biol Chem. 2020 May 8;295(19):6518-6531. doi: 10.1074/jbc.RA119.012026
• A proof-reading mechanism for non-proteinogenic amino acid incorporation into glycopeptide antibiotics.
  Kaniusaite M, Tailhades J, Marschall EA, Goode RJA, Schittenhelm RB, Cryle MJ..
  Chem Sci. 2019 Aug 29;10(41):9466-9482. doi: 10.1039/c9sc03678d
• Exploring the Tetracyclization of Teicoplanin Precursor Peptides through Chemoenzymatic Synthesis.
  Zhao Y, Goode RJA, Schittenhelm RB, Tailhades J, Cryle MJ.
  J Org Chem. 2020 Feb 7;85(3):1537-1547. doi: 10.1021/acs.joc.9b02640.
• The Diiron Monooxygenase CmlA from Chloramphenicol Biosynthesis Allows Reconstitution of β-Hydroxylation during Glycopeptide Antibiotic Biosynthesis.
  Kaniusaite M, Goode RJA, Schittenhelm RB, Makris TM, Cryle MJ.
  ACS Chem Biol. 2019 Dec 20;14(12):2932-2941. doi: 10.1021/acschembio.9b00862
• LFQ-Analyst: An Easy-To-Use Interactive Web Platform To Analyze and Visualize Label-Free Proteomics Data Preprocessed with MaxQuant.
  Shah AD, Goode RJA, Huang C, Powell DR, Schittenhelm RB.
  J Proteome Res. 2020 Jan 3;19(1):204-211. doi: 10.1021/acs.jproteome.9b00496

2019:

• Allelic association with ankylosing spondylitis fails to correlate with human leukocyte antigen B27 homodimer formation.
  Lim Kam Sian TCC, Indumathy S, Halim H, Greule A, Cryle MJ, Bowness P, Rossjohn J, Gras S, Purcell AW, Schittenhelm RB.
  J Biol Chem. 2019 Dec 27;294(52):20185-20195. doi: 10.1074/jbc.RA119.010257.
• Enzymatic Cascade To Evaluate the Tricyclization of Glycopeptide Antibiotic Precursor Peptides as a Prequel to Biosynthetic Redesign.
  Tailhades J, Zhao Y, Schoppet M, Greule A, Goode RJA, Schittenhelm RB, De Voss JJ, Cryle MJ.
  Org Lett. 2019 Nov 1;21(21):8635-8640. doi: 10.1021/acs.orglett.9b03245.
• Ligand-dependent spatiotemporal signaling profiles of the μ-opioid receptor are controlled by distinct protein-interaction networks.
  Civciristov S, Huang C, Liu B, Marquez EA, Gondin AB, Schittenhelm RB, Ellisdon AM, Canals M, Halls ML.
  J Biol Chem. 2019 Nov 1;294(44):16198-16213. doi: 10.1074/jbc.RA119.008685.
• Atmospheric carbon monoxide oxidation is a widespread mechanism supporting microbial survival
  Cordero PRF, Bayly K, Man Leung P, Huang C, Islam ZF, Schittenhelm RB, King GM, Greening C.
  ISME J. 2019 Nov;13(11):2868-2881. doi: 10.1038/s41396-019-0479-8.
• Molecular Basis of Unexpected Specificity of ABC Transporter-Associated Substrate-Binding Protein DppA from Helicobacter pylori.
  Rahman MM, Machuca MA, Khan MF, Barlow CK, Schittenhelm RB, Roujeinikova A.
  J Bacteriol. 2019 Sep 20;201(20):e00400-19. doi: 10.1128/JB.00400-19.
• Kistamicin biosynthesis reveals the biosynthetic requirements for production of highly crosslinked glycopeptide antibiotics.
  Greule A, Izoré T, Iftime D, Tailhades J, Schoppet M, Zhao Y, Peschke M, Ahmed I, Kulik A, Adamek M, Goode RJA, Schittenhelm RB, Kaczmarski JA, Jackson CJ, Ziemert N, Krenske EH, De Voss JJ, Stegmann E, Cryle MJ.
  Nat Commun. 2019 Jun 13;10(1):2613. doi: 10.1038/s41467-019-10384-w.
• Identification of Metabolically Distinct Adipocyte Progenitor Cells in Human Adipose Tissues.
  Raajendiran A, Ooi G, Bayliss J, O'Brien PE, Schittenhelm RB, Clark AK, Taylor RA, Rodeheffer MS, Burton PR, Watt MJ.
  Cell Rep. 2019 Apr 30;27(5):1528-1540.e7. doi: 10.1016/j.celrep.2019.04.010.
• RNA exploits an exposed regulatory site to inhibit the enzymatic activity of PRC2.
  Zhang Q, McKenzie NJ, Warneford-Thomson R, Gail EH, Flanigan SF, Owen BM, Lauman R, Levina V, Garcia BA, Schittenhelm RB, Bonasio R, Davidovich C.
  Nat Struct Mol Biol 2019 Mar;26(3):237-247. doi: 10.1038/s41594-019-0197-y. Epub 2019 Mar 4.
• Suppressing fatty acid uptake has therapeutic effects in preclinical models of prostate cancer.
  Watt MJ, Clark AK, Selth LA, Haynes VR, Lister N, Rebello R, Porter LH, Niranjan B, Whitby ST, Lo J, Huang C, Schittenhelm RB, Anderson KE, Furic L, Wijayaratne PR, Matzaris M, Montgomery MK, Papargiris M, Norden S, Febbraio M, Risbridger GP, Frydenberg M, Nomura DK, Taylor RA.
  Sci Transl Med. 2019 Feb 6;11(478). pii: eaau5758. doi: 10.1126/scitranslmed.aau5758.
• Characterization of the Src-regulated kinome identifies SGK1 as a key mediator of Src-induced transformation.
  Ma X, Zhang L, Song J, Nguyen E, Lee RS, Rodgers SJ, Li F, Huang C, Schittenhelm RB, Chan H, Chheang C, Wu J, Brown KK, Mitchell CA, Simpson KJ, Daly RJ.
  Nat Commun. 2019 Jan 17;10(1):296. doi: 10.1038/s41467-018-08154-1.

2018:

• A Metabolic Checkpoint for the Yeast-to-Hyphae Developmental Switch Regulated by Endogenous Nitric Oxide Signaling
  Koch B, Barugahare AA, Lo TL, Huang C, Schittenhelm RB, Powell DR, Beilharz TH, Traven A.
  Cell Rep. 2018 Nov 20;25(8):2244-2258.e7. doi: 10.1016/j.celrep.2018.10.080.
• Biodosimetric transcriptional and proteomic changes are conserved in irradiated human tissue
  Keam SP, Gulati T, Gamell C, Caramia F, Arnau GM, Huang C, Schittenhelm RB, Kleifeld O, Neeson PJ, Williams SG, Haupt Y.
  Radiat Environ Biophys. 2018 Aug;57(3):241-249. doi: 10.1007/s00411-018-0746-5. Epub 2018 May 30.
• Inhibition of a K9/K36 demethylase by an H3.3 point mutation found in paediatric glioblastoma
  Voon HPJ, Udugama M, Lin W, Hii L, Law RHP, Steer DL, Das PP, Mann JR, Wong LH.
  Nat Commun. 2018 Aug 7;9(1):3142. doi: 10.1038/s41467-018-05607-5.
• Identification of Novel Acinetobacter baumannii Type VI Secretion System Antibacterial Effector and Immunity Pairs.
  Fitzsimons TC, Lewis JM, Wright A, Kleifeld O, Schittenhelm RB, Powell D, Harper M, Boyce JD.
  Infect Immun. 2018 Jul 23;86(8). pii: e00297-18. doi: 10.1128/IAI.00297-18. Print 2018 Aug.
• Helicobacter pylori Outer Membrane Vesicle Size Determines Their Mechanisms of Host Cell Entry and Protein Content.
  Turner L, Bitto NJ, Steer DL, Lo C, D'Costa K, Ramm G, Shambrook M, Hill AF, Ferrero RL, Kaparakis-Liaskos M.
  Front Immunol. 2018 Jul 2;9:1466. doi: 10.3389/fimmu.2018.01466. eCollection 2018.
• Exploring the oncoproteomic response of human prostate cancer to therapeutic radiation using data-independent acquisition (DIA) mass spectrometry.
  Keam SP, Gulati T, Gamell C, Caramia F, Huang C, Schittenhelm RB, Kleifeld O, Neeson PJ, Haupt Y, Williams SG.
  Prostate. 2018 Jun;78(8):563-575. doi: 10.1002/pros.23500. Epub 2018 Mar 9.
• Amyloid-beta-dependent phosphorylation of collapsin response mediator protein-2 dissociates kinesin in Alzheimer's disease.
  Mokhtar SH, Kim MJ, Magee KA, Aui PM, Thomas S, Bakhuraysah MM, Alrehaili AA, Lee JY, Steer DL, Kenny R, McLean C, Azari MF, Birpanagos A, Lipiec E, Heraud P, Wood B, Petratos S.
  Neural Regen Res. 2018 Jun;13(6):1066-1080. doi: 10.4103/1673-5374.233451.
• Proteomic Identification of Interferon-Induced Proteins with Tetratricopeptide Repeats as Markers of M1 Macrophage Polarization.
  Huang C, Lewis C, Borg NA, Canals M, Diep H, Drummond GR, Goode RJ, Schittenhelm RB, Vinh A, Zhu M, Kemp-Harper B, Kleifeld O, Stone MJ.
  J Proteome Res. 2018 Apr 6;17(4):1485-1499. doi: 10.1021/acs.jproteome.7b00828. Epub 2018 Mar 13.
• Proteotranscriptomic Measurements of E6-Associated Protein (E6AP) Targets in DU145 Prostate Cancer Cells.
  Gulati T, Huang C, Caramia F, Raghu D, Paul PJ, Goode RJA, Keam SP, Williams SG, Haupt S, Kleifeld O, Schittenhelm RB, Gamell C, Haupt Y.
  Mol Cell Proteomics. 2018 Jun;17(6):1170-1183. doi: 10.1074/mcp.RA117.000504. Epub 2018 Feb 20.
• AgRP Neurons Require Carnitine Acetyltransferase to Regulate Metabolic Flexibility and Peripheral Nutrient Partitioning.
  Reichenbach A, Stark R, Mequinion M, Denis RRG, Goularte JF, Clarke RE, Lockie SH, Lemus MB, Kowalski GM, Bruce CR, Huang C, Schittenhelm RB, Mynatt RL, Oldfield BJ, Watt MJ, Luquet S, Andrews ZB.
  Cell Rep. 2018 Feb 13;22(7):1745-1759. doi: 10.1016/j.celrep.2018.01.067.
• Determination of the small RNA GcvB regulon in the Gram-negative bacterial pathogen Pasteurella multocida and identification of the GcvB seed binding region.
  Gulliver EL, Wright A, Lucas DD, Mégroz M, Kleifeld O, Schittenhelm RB, Powell DR, Seemann T, Bulitta JB, Harper M, Boyce JD.
  RNA. 2018 May;24(5):704-720. doi: 10.1261/rna.063248.117. Epub 2018 Feb 12.

2017:

• Aurora Kinase B, a novel regulator of TERF1 binding and telomeric integrity.
  Chan FL, Vinod B, Novy K, Schittenhelm RB, Huang C, Udugama M, Nunez-Iglesias J, Lin JI, Hii L, Chan J, Pickett HA, Daly RJ, Wong LH.
  Nucleic Acids Res. 2017 Dec 1;45(21):12340-12353. doi: 10.1093/nar/gkx904.
• Helicobacter pylori chemoreceptor TlpC mediates chemotaxis to lactate.
  Machuca MA, Johnson KS, Liu YC, Steer DL, Ottemann KM, Roujeinikova A.
  Sci Rep. 2017 Oct 26;7(1):14089. doi: 10.1038/s41598-017-14372-2.
• Halogenation of glycopeptide antibiotics occurs at the amino acid level during non-ribosomal peptide synthesis.
  Kittilä T, Kittel C, Tailhades J, Butz D, Schoppet M, Büttner A, Goode RJA, Schittenhelm RB, van Pee KH, Süssmuth RD, Wohlleben W, Cryle MJ, Stegmann E.
  Chem Sci. 2017 Sep 1;8(9):5992-6004. doi: 10.1039/c7sc00460e. Epub 2017 Jul 13.
• Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming.
  Liu X, Nefzger CM, Rossello FJ, Chen J, Knaupp AS, Firas J, Ford E, Pflueger J, Paynter JM, Chy HS, O'Brien CM, Huang C, Mishra K, Hodgson-Garms M, Jansz N, Williams SM, Blewitt ME, Nilsson SK, Schittenhelm RB, Laslett AL, Lister R, Polo JM.
  Nat Methods. 2017 Nov;14(11):1055-1062. doi: 10.1038/nmeth.4436. Epub 2017 Sep 25.
• Structural basis for substrate selection by the translocation and assembly module of the β-barrel assembly machinery.
  Bamert RS, Lundquist K, Hwang H, Webb CT, Shiota T, Stubenrauch CJ, Belousoff MJ, Goode RJA, Schittenhelm RB, Zimmerman R, Jung M, Gumbart JC, Lithgow T.
  Mol Microbiol. 2017 Oct;106(1):142-156. doi: 10.1111/mmi.13757. Epub 2017 Aug 9.
• A Helicobacter pylori Homolog of Eukaryotic Flotillin Is Involved in Cholesterol Accumulation, Epithelial Cell Responses and Host Colonization.
  Hutton ML, D'Costa K, Rossiter AE, Wang L, Turner L, Steer DL, Masters SL, Croker BA, Kaparakis-Liaskos M, Ferrero RL.
  Front Cell Infect Microbiol. 2017 Jun 6;7:219. doi: 10.3389/fcimb.2017.00219. eCollection 2017.
• Chlorinated Glycopeptide Antibiotic Peptide Precursors Improve Cytochrome P450-Catalyzed Cyclization Cascade Efficiency.
  Peschke M, Brieke C, Goode RJ, Schittenhelm RB, Cryle MJ.
  Biochemistry. 2017 Mar 7;56(9):1239-1247. doi: 10.1021/acs.biochem.6b01102. Epub 2017 Feb 24.