While a background in mass spectrometry and/or bioinformatics is beneficial, it is not mandatory and we do consider every student’s interest and skill set to find a suitable project.
All honours (and PhD) projects are highly technology-driven. In essence, they are about establishing, applying or developing state-of-the-art techniques or software scripts to improve, automate and simplify mass spectrometric analyses. These tools will be used and tested on actual research samples from clients and collaborators of our lab. The following projects are just a few examples, which highlight the diversity of the available projects:
Phosphorylation events on amino acids other than serine, threonine and tyrosine are believed to play an important role in cancer cell biology. We would like to establish a robust methodology to study this ‘dark phosphoproteome’ and analyze various cancer cell lines.
MZMine is an open-source software package, which is heavily used by the metabolomics and lipidomics community. We would like to contribute new features and improve existing functions to enhance the functionality of MZMine.
Several methodologies have been developed in the last years to accurately quantify peptides and proteins in complex biological samples such as DIA/SWATH or DDA. We would like to thoroughly compare the performance of these methodologies on multiple mass spectrometers (for example Orbitrap vs TOF) to understand their limitations and benefits