Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) is a powerful technique to study the composition of various samples. It is however particularly promising in the context of diagnostics based on body fluids, such as blood and its derivatives (e.g. serum, plasma).
Our studies of bacteria are focused around a variety of problem. A significant part of them is devoted to issues related to the medical field. Research is centred especially around two major streams: (1) diagnosis and (2) study of mechanisms related to drug resistance.
Studies into DNA conformation and anticancer drug interactions Since Watson and Crick’s discovery of the function of DNA based on Rosalind Franklin’s and Maurice Wilkin’s X-ray diffraction patterns tremendous scientific curiosity has been aroused by the molecule of life.
The Monash Centre for Biospectroscopy is collaborating with the Eliminate Dengue program, part of the Institute of Vector-Borne Diseases based at Monash University. We have been working on a new ATR-FTIR-based method for detecting Wolbachia in adult mosquitos, along with other research-relevant characteristics.
The lack of analytical data tools for integrating information obtained from different platforms makes the holistic comprehension of complex biological systems a challenge. The general aim is to develop new chemometric and multivariate imaging approaches to analyse data obtained from different spectroscopic modalities for the holistic analysis of biological samples.
Raman and infrared microscopes enable imaging of a direct molecular metabolic fingerprint of the sample, providing an alternative to the classical use of fluorescence labelled microscopy. The limitations in spatial resolution, especially in the case of infrared- are being improved by the development of vibrational techniques with resolution in the nano-meter range, such as NanoIR and the Tip enhanced Raman scattering (TERS).