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Aguilar lab publications

Peptide-based drug design | Peptide-based materials | Biomembrane biophysics

Selected highlight publications

1. Luder K, Kulkarni K, Lee HW, Widdop R, Del Borgo MP* & Aguilar MI*. 2016. Decorated Self-Assembling β3- Tripeptides Form Cell Adhesive Scaffolds. Chem Commun 52:4549-4552.

2. Hirst D, Lee TH, Pattenden LK, Thomas WG and Aguilar MI. 2015. Helix 8 of the angiotensin-1a receptor interacts with phosphatidylinositol phosphates and modulates membrane insertion. Scientific Reports 5:09972.

3. Andreu-Fernández V, Genoves A*, Lee TH, Stellato M, Lucantoni F, Orzáez, M, Mingarro, I, Aguilar MI* & PerezPayá, E. 2014. Peptides derived from Transmembrane (TM) domains of diverse Bcl-2 proteins exert disruptive behaviour in mitochondrial membranes. ACS Chem Biol. 9, 1799-1811.

4. Hall, K, Lee TH, Mechler A, Swann MJ and Aguilar MI. 2014. Real-time Measurement of Multiple Membrane Conformational States During Antimicrobial Peptide Binding: The Balance Between Recovery and Lysis. Scientific Reports, June 27 4:5479.

5. Del Borgo MP, Mechler AI, Traore D, Forsyth C, Wilce JA, Wilce MCJ, Aguilar MI* and Perlmutter* P. 2013. Supramolecular Self-Assembly of N-Acetyl capped β-Peptides Leads to Nano-to Macroscale Fibre Formation’. Angewandte Chemie Int Ed., 52 8266- 8270. Selected for Front Piece Highlights.

(See also the publications highlighted for each research area within the Research page)

Additional publications

A full list of Aguilar Lab publications can be viewed on PubMed.


Recent publications – Peptide-based drug design

1. Wang Y, Del Borgo MP, Lee HW, Baraldi D, Hirmiz B, Gaspari TA, Denton KM, Aguilar MI, Samuel CS and Widdop RE, ‘Anti-fibrotic potential of AT2 receptor agonists’, Frontiers in Pharmacol, Vol 8, 564. doi.org/10.3389/fphar.2017.00564.

2. Del Borgo MP, Wang Y, Bosnyak S, Khan M, Walters P, Spizzo I, Perlmutter P, Hilliard L, Denton K, Aguilar MI, Widdop R, Jones ES, Beta-Pro7, ‘Ang III is a novel highly selective angiotensin AT2R agonist, which acts as a vasodepressor agent via the AT2 receptor in conscious SHR’, Clinical Sciences, 129 (2015) 505–513. doi: 10.

3. Watson GM, Kulkarni K, Brandt R, Del Borgo MP, Zhang L, Aguilar MI, Daly RJ and Wilce JA. ‘The full-length Penetratin cell penetrating peptide is required for cytosolic delivery of a Grb7 targeting peptide’. ACS Omega, 2 (2017) 670-677. DOI: 10.1021/acsomega.6b00561.

4. Clayton DJ, Hanchapola I, Thomas WG, Widdop RE, Smith AI, Perlmutter P and Aguilar MI,Structural determinants for binding to angiotensin converting enzyme 2 (ACE2) and angiotensin receptors 1 and 2’, Frontiers In Pharmacology. (2015) Jan 30;6:5. doi: 10.3389/fphar.2015.00005. eCollection 2015.

5. McDonald C, Payne N, Sun G, Clayton DJ, Del Borgo MP, Aguilar MI, Perlmutter P and Bernard C, ‘Single β3-amino acid substitutions to MOG peptides suppress the development of experimental autoimmune encephalomyelitis’, J Neuroimmunology, 277 (2014) 67-76.

Recent publications - Peptide-based materials

1. Christofferson AJ, Al-Garawi Z, Todorova N, Turner J, Del Borgo MP, Serpell LC, Aguilar MI, Yarovsky Y, ‘Identifying the Coiled-Coil Triple Helix Structure of β-Peptide Nanofibers at Atomic Resolution.’ ACS Nano, 12 (2018) 9101-9109. DOI: 10.1021/acsnano.8b03131.

2. McFetridge M, Del Borgo MP, Aguilar MI, Ricardo SD, ‘The Use of Hydrogels for Cell-Based Treatment of Chronic Kidney Disease’, Clinical Sciences 132 (2018)1977-1994.

3. Kulkarni K, Hung JC, Fulcher AJ, AHP Chan, Hong A, Forsythe JS, Aguilar MI, Wise SG* and Del Borgo MP*. ‘β3-tripeptides co-assemble into fluorescent hydrogels for serial monitoring in vivo’, ACS Biomaterials Science and Engineering, 4 (2018) 3843–3847. DOI 10.1021/acsbiomaterials .8b01065.

4. Kulkarni K, Habila N, Del Borgo MP and Aguilar MI*, ‘Novel Materials from the Supramolecular Self-Assembly of Short Helical N-Acetyl-β3-Peptide Foldamers’. Frontiers in Chemistry. 7 (2019) 70.  doi: 10.3389/fchem.2019.00070.

5. Del Borgo MP, Kulkarni K, Tonta MA, Ratcliffe JL, Seoudi R, Mechler AI, Perlmutter P,  Parkington HC and Aguilar MI, 'β3-Tripeptides Act as Sticky Ends to Self-Assemble into a Bioscaffold’, APL Bioengineering, 2 (2018) 026104. DOI: 10.1063/1.5020105. Promoted as a Featured Article.

6. Del Borgo MP, Kulkarni K and Aguilar MI, ‘Using β-Amino Acids and β-Peptide Templates to Create Bioactive Ligands and Biomaterials’, Current Pharmaceutical Design, 23 (2017) 3772-3785. doi: 10.2174/1381612823666170616083031.

7. Del Borgo MP, Kulkarni K, Aguilar MI, ‘Unique Functional Materials Derived from β-Amino Acid Oligomers’, Aust J Chem, 70 (2017) 126–129. DOI. 10.1071/CH16511.

8. Kulkarni K, Motamed S, Habila N, Perlmutter P, Forsythe J, Aguilar MI and Del Borgo MP ‘Orthogonal strategy for the synthesis of dual-functionalised β3 peptide based hydrogels’. Chem Commun, 52 (2016) 5844-5847. DOI: 10.1039/C6CC00624H.

9. Luder K, Kulkarni K, Lee HW, Widdop R, Del Borgo MP* & Aguilar MI*, ‘Decorated Self-Assembling β3-Tripeptides Form Cell Adhesive Scaffolds’. Chem Commun, 52 (2016) 4549-4552. DOI: 10.1039/C6CC00247A.

10. Motamed S, Del Borgo MP, Kulkarni K, Habila N, Zhou K, Perlmutter P, Forsythe J, and Aguilar MI, ‘A self-assembling β-peptide hydrogel for neural tissue engineering”. Soft Matter, 12 (2016) 2243 - 2246. DOI:10.1039/c5sm02902c.

11. Gopalan R, Del Borgo MP, Mechler A, Perlmutter P and Aguilar MI, ‘Geometrically Precise Building Blocks: the Self-Assembly of β-Peptides’, Chemistry & Biology, 22 (2015) 1417-1423.

12. Del Borgo MP, Mechler* AI, Traore D, Forsyth C, Wilce JA, Wilce MCJ, Aguilar MI* and Perlmutter* P, ‘Supramolecular Self-Assembly of N-Acetyl capped β-Peptides Leads to Nano-to Macroscale Fibre Formation’. Angewandte Chemie Int Ed. 2013, 52 8266-8270. DOI: 10.1002/anie.201303175. Selected for Fronticepiece Highlights.

Recent publications – Biomembrane biophysics

Technology development

1. Lee TH, Hirst DJ, Kulkarni K, Del Borgo MP and Aguilar MI, ‘Exploring Molecular-Biomembrane Interactions with Surface Plasmon Resonance and Dual Polarization Interferometry Technology: Expanding the Spotlight onto Biomembrane Structure’, Chemical Reviews, on line May 25, 2018. DOI: 10.1021/acs.chemrev.7b00729.

2. Lee TH and Aguilar MI, ‘Preparation and characterization of supported lipid bilayers for biomolecular interaction studies by dual polarization interferometry’. Advances in Biomembranes and Lipid Self-Assembly, 25 (2017) 125-159. DOI.org/10.1016/bs.abl.2016.12.003.

3. Payne JAE, Lee TH, Anderson MA, Aguilar MI, ‘Binding kinetics of membrane active peptides by Dual Polarisation Interferometry’, Bio-protocols, 7 (2017). DOI 10.21769/BioProtoc.2087.

4. Lee TH., Hall, K, and Aguilar MI, ‘Antimicrobial Peptide Structure and Mechanism of Action: A Focus on the Role of Membrane Structure’, Current Topics in Med Chem, 16 (2016) 25–39. DOI: 10.2174/1568026615666150703121700.

5. Lee TH, Hirst D, and Aguilar MI, ‘New Insights into the Molecular Mechanisms of Biomembrane Structural Changes and Interactions by optical biosensor technology’, BBA Biomembranes, 1848 (2015) 1868–1885. doi: 10.1016/j.bbamem.2015.05.012.

6. Hirst D, Lee TH, Swann MJ, and Aguilar MI, ‘Multi-state kinetic analysis of antimicrobial peptide-membrane interactions’, Anal Chem., 85 (2013) 9296−9304.

Antimicrobial peptide action

1. Lee TH, Hofferek V, Separovic F, Reid G* and Aguilar MI*. ‘The role of bacterial lipid diversity and membrane properties in modulating antimicrobial peptide activity and drug resistance’. Current Opinion in Chemical Biology, Accepted May 27 2019.

2. Lee TH, Sani M-A, Overall S, Separovic F and Aguilar MI, ‘Effect of Phosphatidylcholine Bilayer Thickness and Molecular Order on the Binding of the Antimicrobial Peptide Maculatin 1.1’, BBA Biomembranes, 1860 (2018), 300-309. doi: 10.1016/j.bbamem.2017.10.007.

3. Dekan Z, Baldo B, Donovan G, Lee TH, Aguilar MI, Headey S, Scanlon M, Deuis J, Vetter I, Elliott A, Cooper MA, Alewood D and Alewood PF, ‘Δ-Myrtoxin-Mp1a is a helical heterodimer from the venom of the Jack Jumper ant with antimicrobial, membrane disrupting and nociceptive activities’. Angewandte Chemie, 56 (2017) 8495-8499. doi: 10.1002/anie.201703360.

4. Payne JAE, Bleackley M, Lee TH, Shafey TM, Poon IKH, Hulett MD, Aguilar MI, van der Weerden NL, Anderson MA, ‘The plant defensin NaD1 introduces membrane disorder through a specific interaction with the lipid, phosphatidylinositol 4,5 bisphosphate’, BBA Biomembranes, 1858 (2016), 1099-1109. doi:10.1016/j.bbamem.2016.02.016.

5. Deuis JR, Dekan Z, Inserra MC, Mobli M, Lee TH. Aguilar MI, Craik DJ, Lewis RJ, Alewood PF, Schroeder C, Henriques ST, and Vetter I, ‘Development of a μO-conotoxin analogue with improved lipid membrane interactions and potency for the analgesic target NaV1.8’. J Biol Chem 291 (2016) 11829-11842.doi: 10.1074/jbc.M116.721662.

6. Sani M-A, Lee TH, Aguilar MI* and Separovic F*, ‘Proline-15 creates an amphipathic wedge in maculatin 1.1 peptides that drives lipid membrane disruption’, BBA Biomembranes, 1848 (2015) 2277–2289. doi.org/10.1016/j.bbamem.2015.06.013.

7. Hirst D, Lee TH, Pattenden LK, Thomas WG and Aguilar MI, ‘Helix 8 of the angiotensin-1a receptor interacts with phosphatidylinositol phosphates and modulates membrane insertion’. Scientific Reports (2015). 5:09972, DOI: 10.1038/srep09972.

8. Hall, K, Lee TH, Mechler A, Swann MJ and Aguilar MI, ‘Real-time Measurement of Multiple Membrane Conformational States During Antimicrobial Peptide Binding: The Balance Between Recovery and Lysis’. Scientific Reports, June 27 4:5479 (2014). DOI: 10.1038/srep05479.

9. Lee TH, Heng C, Separovic F and Aguilar MI, ‘Comparison of Reversible Membrane Destabilisation Induced by Antimicrobial Peptides Derived from Australian Frogs’, Biochim Biophys Acta (Biomembranes), 1838 (2014) 2205-15.

Role of the membrane in apoptosis

1. Andreu-Fernández V, Genoves A*,Lee TH,Stellato M, Lucantoni F, Orzáez, M, Mingarro,I, Aguilar MI* & Perez-Payá, E, ‘Peptides derived from Transmembrane (TM) domains of diverse Bcl-2 proteins exert disruptive behavior in mitochondrial membranes’.ACS Chem Biol, 9 (2014) 1799-1811.

2. Suhaili SJ, Karimian H, Stellato M, Lee TH and Aguilar MI, ‘Mitochondrial Outer Membrane Permeabilization: A Focus on the Role of Mitochondrial Membrane Structural Organisation', Biophysical Reviews, 9 (2017) 443-457. doi: 10.1007/s12551-017-0308-0.