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Genome Modification

 

About us

​Welcome to Monash Genome Modification Platform. Our platform can fulfill all your requirements for disease models in biomedical research, including genetically modified mice, rats and other species. Working together with the Australian Phenomics Network (APN) node at Monash, and the Monash Animal Research Platform (MARP), we deliver a complete and comprehensive service in genome modification using CRISPR, embryonic stem cell and transgenic technologies.

Services

CRISPR mutagenesis

Using CRISPR technology, we make null mutations, defined point mutations, epitope and reporter tags, and conditional mutations in mice. We also make null mutations in rats and other species.

Gene targeting in mouse embryonic stem cells

We make genetic modifications in mice using classical gene targeting strategies in embryonic stem cells. This involves the production of chimaeras for obtaining germ line transmission of the genetic modification.

Production of transgenic mice, rats and other species

We make transgenic mice, rats and other species by injecting fertilized eggs with DNA for random integration into the genome. Applications include BAC transgenesis for faithful gene expression, and smaller plasmid constructs for various purposes.

DNA construct design and production

We can design and make your construct for CRISPR mutagenesis, gene targeting in ES cells or transgenic animal production. With our BAC recombineering expertise, there is no limit to the type and range of constructs we can make — quickly and cost effectively.

Other capabilities

Cryopreservation and recovery of genetically modified lines: embryos, sperm

Australian Phenomics Facility

MARP embryo and sperm cryopreservation services

Southern blotting

Design of genotyping protocols: PCR, Southern blot

Importation of genetic resources from international repositories: embryonic stem cells, mice, rats

Pricing

Please download our latest price list

list

PRICE LIST

Resources

Animals

We use use strain C57BL/6JMarp (B6J) as our standard for producing genetically modified mice. This strain was sourced directly from The Jackson Laboratory (JAX), strain C57BL/6J, JAX stock no. 000664. We last re-stocked from JAX in 2012. Our stock remains within 20 generations of brother-sister mating from the JAX strain to minimize genetic drift and to circumvent substrain formation. At JAX, strain C57BL/6J is included in their Genetic Stability Program to curtail genetic drift. Strain C57BL/6N (B6N) is used in conjunction with embryonic stem cells imported from international repositories. Others strains can be used on request — please enquire.

JAX Genetic Stability Program

More researchers are using B6J mice than ever before

The importance of understanding substrains in the genomic age

Team

  • Leanne Hawkey

    Platform Manager

    E | leanne.hawkey@monash.edu

  • Ian Smyth

    Director, Australian Phenomics Network (APN)

    E | ian.smyth@monash.edu

  • Jeff Mann

    Director, MGMP
    Embryonic stem cells, gene targeting

    E | jeff.mann@monash.edu

  • Jeanette Rientjes

    Manager, DNA engineering 
    Construct design and production, BAC recombineering

    E | jeanette.rientjes@monash.edu

  • Tanya Templeton

    Operations Manager, MARP
    Microinjection, cryopreservation 

    E | tanya.templeton@monash.edu

Useful links

Online books

Nomenclature

Other nomenclature