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using CRISPR technology in zygotes to produce a site-specific mutation
Support from the National Collaborative Research Infrastructure Strategy (NCRIS) allows Phenomics Australia to offer Australian Researchers and their collaborators reduced costs for CRISPR projects
microinjection of DNA, resulting in random integration of a foreign gene into the genome
DESCRIPTION OF SERVICE
Price
Rodent production utilising DNA construct (made in-house or provided by client)
mice
7,000
rats
generation of founder chimaeras
breeding to next-generation (heterozygous) to ensure germline transmission of mutation
genotyping each generation by PCR
ddPCR to determine copy number of the transgene
provision of a genotyping strategy
10,500
Please Note: Description of all services involving mouse production are based on use of the C57BL/6J strain.
Other strains may be available but will incur a surcharge, please enquire.
Mutant Cell Lines
Production of a knockout (KO), knockin (KI), point (PM) or conditional knockout (CKO) mutation in a cell line. This can be achieved in one of several ways:
available for Immortalised, Embryonic Stem & induced Pluripotent Stem (iPS) cells (mouse or approved human lines)
Support from the National Collaborative Research Infrastructure Strategy (NCRIS) allows Phenomics Australia to offer Australian Researchers and their collaborators reduced costs for CRISPR projects
DESCRIPTION OF SERVICE
KO
PM*
KI*
Immortalised cell line
3,000
4,200**
6,000
Mouse ES or iPS cell line
3,600
4,800
6,600
Human ES or iPS cell line
4,800
7,200
9,000
* guaranteed PM/KI on one allele, second allele might be a KO
** diploid only cells, please enquire for pricing & feasibility of triploid/polyploid cell lines
