Next Generation Sequencing

MGBP offers a comprehensive suite of DNA sequencing applications to support a wide range of research goals. These include resequencing, de novo genome assembly, gene discovery, and the identification of genomic variation such as SNPs, structural rearrangements, insertions, and deletions.

We provide single-end, paired-end, and synthetic long-read sequencing, with read lengths from 50 to 400 bases and extended coverage reaching kilobases to megabases. Our team can assist in designing and optimising experiments to ensure the most appropriate sequencing strategy for your research objectives.

MGBP also offers a comprehensive quality control service to assess the integrity and suitability of your DNA samples or sequencing libraries prior to library construction, helping to ensure the highest-quality results.

MGBP offers a complete range of RNA sequencing services to support gene expression, transcript discovery, and isoform analysis across diverse sample types. We provide both mRNA-seq for polyadenylated transcripts and total RNA-seq for comprehensive transcriptome profiling, including non-coding and small RNAs.

Our workflows accommodate degraded or low-input samples, enabling analysis from challenging clinical or environmental sources. Using short- and long-read sequencing platforms, we deliver accurate quantification, alternative splicing detection, and full-length transcript reconstruction. Our team can assist with experimental design, rRNA depletion or enrichment strategies, and bioinformatic interpretation to ensure high-quality, biologically meaningful results.

MGBP provides comprehensive metagenomic sequencing services for the analysis of complex microbial communities from environmental, clinical, or host-associated samples. Our workflows enable the unbiased identification and functional profiling of bacteria, archaea, viruses, and eukaryotic microorganisms directly from mixed DNA samples.

We offer both short- and long-read metagenomic sequencing using Illumina,  Oxford Nanopore, and PacBio platforms, enabling detailed insights into taxonomic composition, genomic diversity, and metabolic potential. Our team can assist with project design, library preparation, and bioinformatic analysis, ensuring high-quality data and biologically meaningful interpretation.

MGBP provides targeted 16S and 18S rRNA gene amplicon sequencing for high-resolution profiling of bacterial, archaeal, and eukaryotic microbial communities. These assays enable precise identification and comparison of taxa within complex environmental, clinical, or host-associated samples.

We offer flexible options for amplicon design, primer selection, and read length, supporting both short-read (Illumina) and long-read (Oxford Nanopore and PacBio Vega) platforms to achieve species- or even strain-level resolution. Our team can assist with experimental design, library preparation, and data interpretation, ensuring reproducible and biologically meaningful results tailored to your project.

We offer full-length plasmid sequencing using long-read Oxford Nanopore technology, allowing complete, end-to-end reconstruction of plasmids without fragmentation or tiling PCR. This approach reliably resolves repetitive regions, confirms insert sequence and orientation, and validates overall plasmid structure.

Runs: Weekly (Tuesday)
Turnaround: Results by Friday evening (samples received Wed–Fri run the following week)
Input: 200 ng DNA per sample
Request: Submit via iLab under “Full-length Plasmid/Amplicon Sequencing”
https://monash.ilab.agilent.com/sc/4451/monash-genomics-bioinformatics-platform-micromon-genomics?tab=services