Protein Stability Testing

Differential scanning fluorimetry (DSF) through thermal melting of macromolecules provides a means of testing the stability of samples in a variety of conditions to obtain the optimal buffer that best stabilises each protein for crystallisation.

DSF is also useful for detecting and confirming ligand and small molecule binding to macromolecules through a change to the melting behaviour, and can be used as a step in fragment or compound screening for drug development.

Our Prometheus NT.48 (NanoTemper) can measure protein stability through a change in the intrinsic fluorescence emission of aromatic residues as a sample is heated up. It can also detect aggregation of a sample through backscattering.