About
The Monash Ramaciotti Centre for Cryo-Electron Microscopy (Cryo-EM) is a leading research facility for biological electron microscopy.
Our platform provides expertise in advanced electron microscopy for biomedical discoveries at the molecular and cellular level. We offer a range of biological EM (Bio-EM) techniques from scanning electron microscopy (SEM) and transmission electron microscopy (TEM) to immuno electron microscopy, correlative light and electron microscopy, Cryo-tomography and structure determination using single particle Cryo-EM.
We maintain a suite of advanced electron microscopes, including Australia’s first Titan KRIOS – currently the most powerful microscope for Bio-EM.
Expertise
Our platform supports researchers by developing and applying advanced imaging techniques to study the life sciences.
We conduct research into advanced Bio-EM techniques. The team also provides training and expertise to Australian and international collaborators on advanced EM projects.
Working With Us
Specialist Services
Transmission Electron Microscopy
Immuno Electron Microscopy
Scanning Electron Microscopy
Structural Cryo-EM
Single Particle Analysis
Correlative Microscopy
Microscopes
FEI Titan Krios Cryo-TEM
The FEI Titan Krios Cryo-TEM has an autoloader for 12 Cryo samples, a Falcon III Direct Electron Detector, a Gatan Energy Filter with a K2 Summit Direct Electron Detector, and a Volta Phase Plate.
FEI Tecnai Spirit G2 TEM
The FEI Tecnai Spirit is a 120keV Cryo-capable TEM. Holders include Gatan 626 Cryo transfer holder and Fishione tomography holder.
FEI Nova NanoSEM
The FEI Nova NanoSEM is equipped with secondary electron, backscatter, and STEM detectors. The microscope runs the correlative software package MAPS.
JEOL JEM-1400PLUS TEM
The JEOL 1400 Plus, a 120keV TEM, is used for standard cell and tissue samples and negatively stained proteins.
FEI Helios G4 UX cryo-FIBSEM
The new FEI Helios G4 UX is equipped with a Leica VCT500 cryo-stage, EasyLift NanoManipulator, and Oxford EDS system. Software for Slice & View, CLEM, TEM lamellae preparation, 3D EDS analysis.
FEI Talos Arctica Cryo-TEM
The 200keV FEI Talos Arctica Cryo-TEM has a 12 cryo-grid autoloader, a Falcon III direct electron detector and a Volta phase plate.
Sample Preparation Equipment
- Ultramicrotomes
We have two Leica UCT and one UCS Ultramicrotomes in the lab.
- Cryo-Ultramicrotomes
The facility has two Leica UC7/FC7 Cryo-Ultramicrotomes.
- High-Pressure Freezer
We have a Leica EMPact2 and a Leica AFS2 for automatic freeze substitution.
- Plunge Freezer
Two FEI Vitrobots Mark IV for Cryo preparation.
- Coating
Gold, carbon, and platinum coating: Leica ACE200, ACE600, and SCD005.
- Critical Point Drying
Baltec CPC030.