BD Influx

  • Lasers 4: 405nm, 488nm, 561nm, 635nm
  • Detectors: 15 fluorescent channels
  • Sterile sorting
  • Index sorting
  • Software: Sortware
  • Sorting: up to 6 ways, up to 384 well plates
  • Sample tubes: 5ml Polypropylene
  • Nozzles: 70µm, 85µm and 100µm
  • Potential Maximum Speeds: 70µm 25,000 cells/sec, 85µm 10,000 cells/second, 100µm 5,000 cells/sec
  • BD Influx Detector Guide

Laser Wavelength

Filter

Mirror

Frequently Used Fluorochromes

Blue 488nm

530/40

505 LP

FITC, GFP, A488, CFSE, BB515

 

670/30

550 LP

PerCP, PI, 7AAD

 

710/50

685 LP

PerCP-Cy5.5, PerCP-eF710, QD705, 7AAD, PI

Red 640nm

670/30

650 LP

APC, A647, Cy5

 

720/40

700 LP

A680, A700

 

750 LP

740 LP

APC-Cy7. APC-eF780, APC-Fire 780

Violet 405nm

460/50

  •  

BV421, Pac Blue. Sytox Blue, e450

 

520/35

  •  

BV500, BV510, Aqua, Pac Orange

*Only two can be used


together*

*610/20

550 LP

BV605, e605, QD605

*660/20

645 LP

BV655, QD655

*710/50

685 LP

BV711, QD705

*750 LP

740 LP

BV785

Yellow-Green 561nm

585/29

570 LP

PE, A546, RFP, DS-Red, dTomato

 

610/20

600 LP

PE-CF594, PE-TexRed, A594, mCherry, PI

 

670/30

645 LP

PE-Cy5, PE-Cy5.5, PI

 

710/50

685 LP

PE-Cy5

 

750 LP

740 LP

PE-Cy7, PE-Vio 770, PE-A750, QD800