Amnis ImageStreamX MkII
- Lasers 4: 405nm, 488nm, 642nm, 785nm
- Detectors: 10 fluorescent channels
- Channels: 12
- Brightfield: 2
- Cameras: 2
- Sample tubes: 1.5ml microcentrifuge tubes
- Software: Inspire
- AMNIS ImageStreamX MKII Detector Guide
Laser Wavelength | ||||||
Camera | Channel | Filter | Violet 405nm | Blue 488nm | Red 642nm | SSC 785nm |
1 | 1 | 457/45 | Brightfield | |||
2 | 528/65 | FITC, GFP | ||||
3 | 577/35 | PE, Cy3 | ||||
4 | 610/30 | 7AAD, PI | ||||
5 | 702/85 | PerCP | ||||
6 | 762/35 | PE-Cy7 | SSC | |||
2 | 7 | 457/45 | DAPI, Ho | |||
8 | 537/65 | BV510 | ||||
9 | 582/25 | eF585 | ||||
10 | 610/30 | BV605 | ||||
11 | 702/85 | BV711 | APC, A700 | |||
12 | 762/35 | BV785 | APC-Cy7 | SSC | ||