BD LSR II
- Lasers 4: 405nm, 488nm, 561nm, 640nm
- Detectors: 16 fluorescent channels
- Sample tubes: 5ml Polystyrene
- Software: FACSDiva
- Fast analysis rates >20,000 events per second
- BD LSRII Detector Guide
Laser Wavelength | Filter | Mirror | Frequently Used Fluorochromes |
Red 640nm | 670/14 | APC, A647, Sytox Red | |
730/45 | 690 LP | A680, A700 | |
780/60 | 750 LP | APC-Cy7. APC-eF780, APC-Fire 780, NIR | |
Blue 488nm | 530/30 | 505 LP | FITC, GFP, A488, CFSE, BB515 |
710/50 | 630 LP | PI, PerCP, PerCP-eF710, 7AAD | |
Violet 405nm | 450/50 | BV421, Pac Blue. Sytox Blue, e450 | |
525/50 | 505 LP | BV500, BV510, Aqua, Pac Orange | |
586/15 | 570 LP | Pac Orange, QD565, QD585, BV570 | |
610/20 | 600 LP | BV605, eF605, QD605 | |
660/20 | 630 LP | BV650, QD655, eVolve655 | |
710/50 | 685 LP | BV711, QD705 | |
780/60 | 750 LP | BV786, QD800 | |
Yellow-Green 561nm | 582/15 | PE, A546, RFP, DS-Red, dTomato | |
610/20 | 600 LP | PE-CF594, PE-TexRed, A594, mCherry, PI | |
685/35 | 635 LP | PE-Cy5, PE-Cy5.5, PI | |
780/60 | 750 LP | PE-Cy7, PE-Vio 770, PE-A750, QD800 |