BD LSRFortessa X-20

  • Lasers 5: 355nm, 405nm, 488nm, 561nm, 640nm
  • Detectors: 18 fluorescent channels
  • Sample tubes: 5ml Polystyrene
  • High-throughput sampler (HTS): 96 - 384 well plates
  • Software: FACSDiva
  • Fast analysis rates >20,000 events per second
  • BD LSRFortessa X-20 Detector Guide

Laser Wavelength

Filter

Mirror

Frequently Used Fluorochromes

Blue 488nm

530/30

505 LP

FITC, GFP, A488, CFSE, BB515

 

710/50

685 LP

PI, PerCP, PerCP-eF710, 7AAD

Red 640nm

670/30

  •  

APC, A647, Cy5

 

730/45

690 LP

A680, A700

 

780/60

750 LP

APC-Cy7. APC-eF780, APC-Fire 780

Yellow-Green 561nm

586/15

  •  

PE, A546, RFP, DS-Red, dTomato

 

610/20

600 LP

PE-CF594, PE-TexRed, A594, mCherry, PI

 

675/50

635 LP

PE-Cy5, PE-Cy5.5, PI

 

780/60

750 LP

PE-Cy7, PE-Vio 770, PE-A750, QD800

Ultraviolet 355nm

379/28

  •  

BUV395, eF455UV, Hoechst, DAPI, A350

 

525/50

505 LP

BUV496, QD525

 

740/35

690 LP

BUV737, QD800

Violet 405nm

450/50

  •  

BV421, Pac Blue. Sytox Blue, e450

 

525/50

505 LP

BV500, BV510, Aqua, Pac Orange

 

610/20

600 LP

BV605, eF605, QD605

 

670/30

635 LP

BV650,  QD655

 

710/50

685 LP

BV711, QD705

 

780/60

750 LP

BV785, QD800