We can isotype and sequence mouse or rat antibodies from hybridoma cell lines generated in MADP as well those from external sources. Knowing the sequence of your mAb is essential for antibody engineering or for IP protection.
Surface plasmon resonance (SPR) binding analysis is used to study molecular interactions. The technique avoids the need to label either of the interacting species, and the binding event is visualized in real time. SPR can provide concentration and affinity data along with association and dissociation rate constants for the affinity interaction.
The antibody sample is run on a gel to identify the heavy and light chains. The sample is then digested with three different enzymes analysed by LCMS and the resulting data processed using PeaksAB software.
In MADP we need a small amount of supernatant or purified antibody to identify the
common rat or mouse antibody isotypes (IgG 1 , IgG 2a , IgG 2b , IgG 3 , IgM, and IgA), and the light
chains (kappa or lambda).