Phage Antibody Library

Phage Display is a powerful technology to generate antibodies against any target including conformation specific, non-immunogenic or even toxic antigens.
By using a highly diverse human library, fully human antibodies with high affinity can be generated after only a few rounds of panning Monash has now established its first in-house phage library (MOptiFAB) which has already yielded several interesting antibodies.

Monash University’s new proprietary human antibody phage library

Recombinant antibodies currently attract a large share of pharmaceutical research and development investment, and therapeutic success can be seen in various areas of medicine. Phage-Display, awarded the 2018 Nobel Prize in Chemistry, is a powerful technology to generate novel and highly specific antibodies against a range of important targets using fully human antibody libraries.

To give Monash researchers the ability to use Phage-Display in house and generate new antibodies against their targets of interest, the Monash Phage Working Group has developed the University’s first fully owned, proprietary human Phage library. Using advanced bioinformatics and harnessing decades of combined experience in antibody development and Phage-Display, the group worked on the library design and then engaged with Twist to synthesise each clone individually using Twist’s high throughput DNA synthesis technology. The library has now been successfully validated against several targets and it will be available to all Monash researchers for internal use very soon.

Phage-Display is a highly versatile technology to generate antibodies, especially against difficult targets such as confirmation specific epitopes. Monash’s fully owned Phage library gives its researchers a unique tool to make antibodies targeting a range of highly interesting antigens across all major disease indications.

Please note at the current time this library is only available to Monash staff and students.