mAb Services
The Monash Antibody Technologies Facility (MATF) provides services for antibody production, assay development, robotics, high throughput screening and antibody characterisation. We use state-of-the-art equipment to support researchers not only from Monash University but elsewhere in Victoria, Australia and world-wide.
The primary focus in the platform is generating high-affinity monoclonal antibodies using advanced discovery techniques and a flexible, personalised approach to each project. Additionally, we are keen to help customers with antibody or laboratory automation challenges. We are happy to develop new antibody screens and encourage our customers to bring their requests to us.
The staff from MATF work with our customers at every step to ensure the needs of each individual project are met and we are proud to follow a Quality Management System that is certified to ISO9001:2015.
The MATF Platform operates on a fee for services basis for all services and laboratory access.
Custom monoclonal antibody production
Our platform produces hybridoma cell lines expressing monoclonal antibodies against a custom target protein or peptide. Using high throughput flow cytometry or ELISA screening we identify specific IgG secreting cells. These can then be sub-cloned to provide monoclonal antibody producing cell lines. We can perform these services with as little as 1mg of protein or peptide and deliver individual clones and antibody supernatants with a typical production time of 12–16 weeks.
Custom ELISA development
MATF is able to determine the most appropriate complimentary pairs and orientation for the development of a Sandwich ELISA for the detection of your antigen using Surface Plasmon Resonance (SPR) technology.
Cell Banking and Long Term Storage
Our team will cryopreserve your hybridoma cell lines and keep them in monitored liquid nitrogen tanks for safe storage.
Hybridoma Supernatant Generation
From either a growing culture or a frozen vial we can wean your cells into hybridoma serum free media and generate supernatant for you to use in your lab experiments or to purify your monoclonal antibody in your own lab.
Mycoplasma Testing
Routine testing for mycoplasma is recommended to ensure the purity of cultured cells. Mycoplasmas can drastically alter your cells and consequently, affect your research results. We can test your cell lines foro mycoplasma using either a commercial kit or a PCR reaction.
Hybridoma Sub-Cloning
Following identification of a positive sample sub-cloning separates the antigen-specific cell from other cells in the well and generates a line that is that is chromosomally stable. We use single cell deposition by a flow cytometer and microscopy to confirm single cells to generate monoclonal cell lines.