Cross-linking mass spectrometry (CX-MS)

Identifies interacting domains within proteins and protein complexes!

Chemical cross-linker reagents can be used to covalently link adjacent domains within proteins and protein complexes. Identifying the cross-linked domains/peptides by high-resolution mass spectrometry provides a powerful tool to reveal interacting regions of protein complexes. CX-MS can also be used to analyze naturally occurring crosslinks such as disulphide bridges.