MCEM Hands-On Training Courses
MCEM offers a range of courses, some of which are necessary to complete successfully before gaining access to the electron microscopes.
- Introduction to Scanning Electron Microscopy: Theory and Applications in Physical Sciences
- Fundamentals of Dual Beam Microscopy: Applications on a Quanta 3D FEG
- Fundamentals of Electron Backscatter Diffraction (EBSD)
- Introduction to Transmission Electron Microscopy
- Introduction to Crystallography in Electron Microscopy
- Introduction to Electron Diffraction in the TEM
Your training needs will be identified during your initial New Project Interview.
Introduction to Scanning Electron Microscopy: Theory and Applications in Physical Sciences (Required to access MCEM SEMs)
The scanning electron microscope (SEM) basic training course comprises both theory and hands on training, beginning with a 15 minute open book test based on the instrument's user guide carried out using the Monash MOODLE online system.
The first day of training begins with 6 hours of lectures on SEM theory, sample preparation and X-ray generation. This is followed by 2 x 3 hour "hands-on" sessions working in groups of 2, covering basic operation through to optical system alignment. Training continues with 1 hour of one-on-one training, reinforcing the previous work including secondary electron (SE) imaging, back-scattered electron (BSE) imaging and X-ray analysis. The 1 hour practical test follows, in addition to a 30 minute closed book written test based on the study notes and previous lecture.
After successfully completing the practical and theoretical test, a final 1 hour session is booked with the trainer to observe the user's sample and determine optimal operating conditions.
Introduction to Transmission Electron Microscopy (Required to access MCEM TEMs)
All new TEM users start with Tecnai T20 TEM training.
TEM Training Prerequisites: TEM applicants will need to take a short online general background course (at undergraduate level) and pass a CLOSED BOOK multiple-choice quiz using the Monash MOODLE online system.
Trainees attend a TEM induction typically held on a Wednesday. This includes a 3 hour lecture in the morning, followed by 1.5 hours in the lab in the afternoon. Following the TEM induction trainees must pass a 15 minute open book test based on the instrument's user guide carried out using MOODLE.
Practical training is completed on the trainees samples. Practical training is divided into two parts.
- Part One: Sample evaluation and basic training which can be 1 to 4 x 2 hours sessions.
- Part Two: CORE business hours training, 3-6 x 2 hour sessions.
During these sessions practical competence will be assessed, ensuring safe and effective use of the TEM can be made.
Following successful completion of the practical training a 30 minute closed book test on Moodle is to be completed.
Specimen Preparation Training
Specimen preparation for microscopy can be a very complex and time-consuming process. The number of training sessions required may range from one or two for the simpler techniques, to five or more for the difficult techniques.