TriFoil FLECT and Mediso CT Small Animal Imaging System

The TriFoil FLECT and Mediso CT small animal imaging system is located at the Monash Institute for Pharmaceutical Sciences (MIPS - Parkville).

Benefits of FLECT and CT

  • In vivo optical imaging with uniform sensitivity and resolution throughout the object
  • Images of deep tissue phenomena without loss of image quality or quantitative accuracy
  • X-ray CT provides co-registered images for anatomical correlation that significantly improve the segmentation and resolution of the optical images


  • Imaging of rodent animal models with axial and transaxial fields of view of 38mm and 100mm (mice)
  • Full true 3-dimensional tomographic imaging of fluorescent NIR dyes
  • Measures near infrared fluorescence inside a small animal (typically a mouse).
  • Works best in hairless or white mice
  • No radioactivity required for imaging
  • Measure cells labelled with NirFP

Research applications

  • Oncology
  • Immunology
  • Tracking near-infrared dyes (NIR) in vivo
  • Cell trafficking - NirFP

FLECT and CT technical specifications

  • 42 frequency excitation options
  • Up to 16 light filtration options
  • FLECT system capable of imaging in the range of ~500-870 nm
  • Individualised animal tissue heterogeneity map for compensation/correction of attenuation of photons based on CT image
  • Deep tissue algorithms for fluorescence recovery based on individualised geometry of each animal
  • Ability to use plug-in imaging cells compatible with MRI, and to fuse FLECT, CT and MRI images
  • Configured with 'InVivoScope' control and analysis software

Additional support

Our Preclinical Support Team can provide valuable guidance and our Administrative Team can help you navigate the relevant procedures.

Research using MBI's FLECT-CT

The following publications list is of research using MBI's FLECT technology:

Guilleminault et al., Journal of Controlled Release 2014; 196:344-354
Fate of inhaled monoclonal antibodies after the deposition of aerosolized particles in the respiratory system

Htun et al., Nat. Comm. 2017; 8(75):1-16
Near-infrared autofluorescence induced by intraplaque hemorrhage and heme degradation as marker for high-risk atherosclerotic plaques

Lim et al., Theranostics 2017; 7(5):1047-1061
A unique recombinant fluoroprobe targeting activated platelets allows in vivo detection of arterial thrombosis and pulmonary embolism using a novel three-dimensional fluorescence emission computed tomography (FLECT) technology

Yap et al., Theranostics 2017; 7(10):2565- 2574
Targeting activated platelets: a unique and potentially universal approach for cancer imaging

Lisitskiy et al., Bioorg. Med. Chem. Lett. 2017; 27(16):3925-3930
Multifunctional human serum albumin-therapeutic nucleotide conjugate with redox and pH-sensitive drug release mechanism for cancer theranostics

Popova et al., Bioorg. Med. Chem. Lett. 2018; 28(3):260-264
Biotin-decorated anti-cancer nucleotide theranostic conjugate of human serum albumin: Where the seed meets the soil?

Sun et al., Biomaterials 2018; 183:268-279
A targeting theranostics nanomedicine as an alternative approach for hyperthermia perfusion

Hu et al., J. Mater. Chem. B, 2018, 6:6122-6132
Targeted dual-mode imaging and phototherapy of tumors using ICG-loaded multifunctional MWCNTs as a versatile platform