Malvern Zetasizer Nano

Measurement of protein size, electrophoretic mobility of proteins, zeta potential of colloids and nanoparticles

Narrow band filter installed to improve the signal/noise ratio for nanoparticles having fluorescence such as protein particles and quantum dots.

Particle size and molecular size

  • Measurement range: 0.3nm – 10.0 microns
  • Measurement principle: Dynamic Light Scattering
  • Minimum sample volume: 12µL
  • Accuracy: Better than +/-2% on NIST traceable latex standards
  • Precision / Repeatability: Better than +/-2% on NIST traceable latex standards
  • Sensitivity: 0.1mg/mL (Lysozyme)

Zeta potential

  • Measurement range: 3.8nm – 100 microns (diameter)
  • Measurement principle: Electrophoretic Light Scattering
  • Minimum sample volume: 150µL (20µL using diffusion barrier method)
  • Accuracy: 0.12µm.cm/V.s for aqueous systems using NIST SRM1980 standard reference material
  • Sensitivity: 10mg/mL (BSA)

Molecular weight

  • Measurement range: 980Da – 20M Da
  • Measurement principle: Static Light Scattering using Debye plot
  • Minimum sample volume: 12µL (3-5 sample concentrations required)
  • Accuracy: +/- 10% typical